Dnp-RPLALWRS is a fluorescent peptide substrate specifically tailored for human matrilysin (MMP-7). Upon enzymatic cleavage at the alanine-leucine bond, the release of the Dnp group enhances fluorescence by alleviating quenching, allowing for real-time quantitative assessment of MMP-7 activity through increased tryptophan emission. This substrate serves as a sensitive and effective tool for conducting kinetic studies and screening for enzyme inhibitors.
Dnp-RPLALWRS is a fluorescent peptide substrate specifically tailored for human matrilysin (MMP-7). Upon enzymatic cleavage at the alanine-leucine bond, the release of the Dnp group enhances fluorescence by alleviating quenching, allowing for real-time quantitative assessment of MMP-7 activity through increased tryptophan emission. This substrate serves as a sensitive and effective tool for conducting kinetic studies and screening for enzyme inhibitors.
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