Fig. 1 · Structure
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Fluo-3FF pentapotassium
Fluorochrome
Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator that targets cytosolic calcium levels. As a di-fluorinated analog of Fluo-3, Fluo-3FF exhibits a significantly lower calcium affinity (Kds=42 μM) compared to Fluo-3, making it suitable for applications involving compartments with elevated calcium concentrations, such as the endoplasmic reticulum. This unique characteristic allows researchers to monitor and study luminal fluctuations in these high-calcium environments with greater sensitivity.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 348079-14-1 |
| Applications | Fluorochrome |
| Formula | C35H21Cl2F2K5N2O13 |
| Molecular Weight | 981.94 |
| SMILES | O=C1C=C2OC3=C(C(C4=CC(OCCOC5=C(C(F)=CC=C5N(CC(O[K])=O)CC(O[K])=O)F)=C(N(CC(O[K])=O)CC(O[K])=O)C=C4)=C2C=C1Cl)C=C(Cl)C(O[K])=C3 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.