Fig. 1 · Structure
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H2DCFDA
ROS-Sensitive Probe
H2DCFDA is a cell-permeable probe designed for the detection of intracellular reactive oxygen species (ROS). This fluorescent indicator exhibits excitation and emission maxima at 488 nm and 525 nm, respectively. H2DCFDA is widely utilized in research applications involving oxidative stress and cellular signaling, providing insights into ROS-related biological processes.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 4091-99-0 |
| Applications | ROS-Sensitive Probe |
| Formula | C24H16Cl2O7 |
| Molecular Weight | 487.29 |
| Synonyms | DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate |
| SMILES | O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(OC(C)=O)=C4)C=C(OC(C)=O)C(Cl)=C3 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.