Mca-SEVNLDAEFK(Dnp) is a fluorescent substrate peptide specifically designed for the estimation of Beta-secretase 1 (BACE-1) activity. Featuring the 'Swedish' mutation of the amyloid precursor protein (APP) β-secretase cleavage site, this substrate undergoes cleavage at the -Leu-Asp- bond, releasing the highly fluorescent 7-methoxycoumarin (Mca) fragment. This release results in a significant increase in fluorescence intensity due to the removal of the quenching effect from the 2,4-dinitrophenyl (Dnp) moiety, making it a valuable tool for research applications in Alzheimer’s disease and related studies of amyloid pathology.
Mca-SEVNLDAEFK(Dnp) is a fluorescent substrate peptide specifically designed for the estimation of Beta-secretase 1 (BACE-1) activity. Featuring the 'Swedish' mutation of the amyloid precursor protein (APP) β-secretase cleavage site, this substrate undergoes cleavage at the -Leu-Asp- bond, releasing the highly fluorescent 7-methoxycoumarin (Mca) fragment. This release results in a significant increase in fluorescence intensity due to the removal of the quenching effect from the 2,4-dinitrophenyl (Dnp) moiety, making it a valuable tool for research applications in Alzheimer’s disease and related studies of amyloid pathology.
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