Methyltetrazine-amido-bis-(carboxyethoxymethyl)-methane is a versatile click chemistry reagent designed for biochemical assays, featuring three carboxylic acid groups and a methyltetrazine moiety. It facilitates the formation of stable covalent bonds with TCO-containing compounds through a highly selective inverse-electron demand Diels-Alder cycloaddition reaction, eliminating the need for catalysis or elevated temperatures. Additionally, the terminal carboxylic acid can efficiently couple with primary amines in the presence of activators such as EDC or HATU, forming stable amide bonds for a variety of bioconjugation applications in chemical and biological research.
Methyltetrazine-amido-bis-(carboxyethoxymethyl)-methane is a versatile click chemistry reagent designed for biochemical assays, featuring three carboxylic acid groups and a methyltetrazine moiety. It facilitates the formation of stable covalent bonds with TCO-containing compounds through a highly selective inverse-electron demand Diels-Alder cycloaddition reaction, eliminating the need for catalysis or elevated temperatures. Additionally, the terminal carboxylic acid can efficiently couple with primary amines in the presence of activators such as EDC or HATU, forming stable amide bonds for a variety of bioconjugation applications in chemical and biological research.
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