NAD Synthetase is an enzyme that catalyzes the final step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). This enzyme demonstrates differing Km values for its substrates, with values of 200 μM for NAD, 11 μM for ATP, and 0.65 μM for ammonia in Escherichia coli, and 170 μM for NAD, 190 μM for ATP, and 64 μM for ammonia in yeast. NAD Synthetase is applicable for enzymatic assays targeting ATP, ammonia, urea, or creatinine, and is suitable for enzymatic cycling methods. Furthermore, it holds promise for advancing research in metabolic diseases, cancer, aging, and neurodegenerative disorders.
NAD Synthetase is an enzyme that catalyzes the final step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). This enzyme demonstrates differing Km values for its substrates, with values of 200 μM for NAD, 11 μM for ATP, and 0.65 μM for ammonia in Escherichia coli, and 170 μM for NAD, 190 μM for ATP, and 64 μM for ammonia in yeast. NAD Synthetase is applicable for enzymatic assays targeting ATP, ammonia, urea, or creatinine, and is suitable for enzymatic cycling methods. Furthermore, it holds promise for advancing research in metabolic diseases, cancer, aging, and neurodegenerative disorders.
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