REF1 peptide is a potent PORK1 agonist with an EC50 of 0.028 nM in tomato. By binding to the extracellular domain of PORK1, REF1 peptide triggers receptor autophosphorylation, leading to the activation of downstream MPK3/MPK6 signaling pathways involved in defense and regeneration. This compound promotes callus formation and, with prolonged exposure, inhibits shoot regeneration, while enhancing regeneration and transformation efficiency in both recalcitrant dicotyledonous and monocotyledonous crops.
REF1 peptide is a potent PORK1 agonist with an EC50 of 0.028 nM in tomato. By binding to the extracellular domain of PORK1, REF1 peptide triggers receptor autophosphorylation, leading to the activation of downstream MPK3/MPK6 signaling pathways involved in defense and regeneration. This compound promotes callus formation and, with prolonged exposure, inhibits shoot regeneration, while enhancing regeneration and transformation efficiency in both recalcitrant dicotyledonous and monocotyledonous crops.
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