5-CFDA is a fluorescent dye that enables cellular imaging through its mechanism of free diffusion into cells. Once inside, it is hydrolyzed by intracellular non-specific lipases to yield carboxyl fluorescein (CF), which bears an additional negative charge, enhancing its cellular retention compared to standard fluorescein dyes. This compound is widely used in biological research for tracing cellular uptake and monitoring cellular activity in various applications.
5-CFDA is a fluorescent dye that enables cellular imaging through its mechanism of free diffusion into cells. Once inside, it is hydrolyzed by intracellular non-specific lipases to yield carboxyl fluorescein (CF), which bears an additional negative charge, enhancing its cellular retention compared to standard fluorescein dyes. This compound is widely used in biological research for tracing cellular uptake and monitoring cellular activity in various applications.
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