5-FITC tyramide is a green fluorescent dye that combines fluorescein isothiocyanate (FITC) with tyramide, exhibiting excitation at 488 nm and emission at 525 nm. This compound serves as a reporter substrate in horseradish peroxidase (HRP)-catalyzed processes, facilitating tyramide signal amplification (TSA). 5-FITC tyramide is particularly effective in multiplex immunohistochemistry (mIHC), enabling enhanced sensitivity and specificity in detecting multiple antigens within tissue samples.
5-FITC tyramide is a green fluorescent dye that combines fluorescein isothiocyanate (FITC) with tyramide, exhibiting excitation at 488 nm and emission at 525 nm. This compound serves as a reporter substrate in horseradish peroxidase (HRP)-catalyzed processes, facilitating tyramide signal amplification (TSA). 5-FITC tyramide is particularly effective in multiplex immunohistochemistry (mIHC), enabling enhanced sensitivity and specificity in detecting multiple antigens within tissue samples.
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