Calcein AM is a cell-permeable, non-fluorescent dye that readily enters live cells. Once inside, it is hydrolyzed by intracellular esterases to generate Calcein, a polar and highly negatively charged molecule that is retained within the cytoplasm. Calcein emits strong green fluorescence (Ex/Em: 494/515 nm) and is commonly used in combination with Propidium Iodide for assessing cell viability and cytotoxicity.
Calcein AM is a cell-permeable, non-fluorescent dye that readily enters live cells. Once inside, it is hydrolyzed by intracellular esterases to generate Calcein, a polar and highly negatively charged molecule that is retained within the cytoplasm. Calcein emits strong green fluorescence (Ex/Em: 494/515 nm) and is commonly used in combination with Propidium Iodide for assessing cell viability and cytotoxicity.
Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
This calculator helps you calculate mass of compound based on solution concentration, volume and molecular weight in a specific solution using the formula:
