DMNPE-caged D-luciferin is a caged fluorescent dye that serves as a natural substrate for luciferase, an enzyme pivotal for monitoring cellular activity. Upon deprotection, this compound's luminescent properties allow for the emission of yellow-green light at a peak wavelength of approximately 530 nm, contingent on the presence of ATP. Its ability to permeate cell membranes enhances its utility in various biological research applications, including live-cell imaging and bioluminescence-based assays.
DMNPE-caged D-luciferin is a caged fluorescent dye that serves as a natural substrate for luciferase, an enzyme pivotal for monitoring cellular activity. Upon deprotection, this compound's luminescent properties allow for the emission of yellow-green light at a peak wavelength of approximately 530 nm, contingent on the presence of ATP. Its ability to permeate cell membranes enhances its utility in various biological research applications, including live-cell imaging and bioluminescence-based assays.
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