Ethidium bromide is a fluorescent intercalating agent widely used in molecular biology as a nucleic acid stain. It inserts between DNA base pairs, allowing visualization of DNA and RNA under ultraviolet (UV) light during techniques such as agarose gel electrophoresis. Due to its high sensitivity and strong fluorescence, Ethidium bromide is a standard tool for nucleic acid detection, though it is handled with caution because of its mutagenic properties.
Ethidium bromide is a fluorescent intercalating agent widely used in molecular biology as a nucleic acid stain. It inserts between DNA base pairs, allowing visualization of DNA and RNA under ultraviolet (UV) light during techniques such as agarose gel electrophoresis. Due to its high sensitivity and strong fluorescence, Ethidium bromide is a standard tool for nucleic acid detection, though it is handled with caution because of its mutagenic properties.
Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 1 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
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