Fluorescein-6-isothiocyanate is a fluorescent dye that primarily targets amine groups in proteins through its isothiocyanate functionality. It exhibits high binding affinity with dissociation constants (Kds) of 8.74, 2.72, and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG, and 4-4-20 antibodies, respectively. This compound is widely used in imaging applications, including cellular labeling and immunohistochemistry, facilitating the detection and visualization of target biomolecules in research settings.
Fluorescein-6-isothiocyanate is a fluorescent dye that primarily targets amine groups in proteins through its isothiocyanate functionality. It exhibits high binding affinity with dissociation constants (Kds) of 8.74, 2.72, and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG, and 4-4-20 antibodies, respectively. This compound is widely used in imaging applications, including cellular labeling and immunohistochemistry, facilitating the detection and visualization of target biomolecules in research settings.
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