Fig. 1 · Structure

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Fluorescein-maleimide

Fluorescein Derivative

Fluorescein-maleimide is a thiol-reactive fluorescent derivatization reagent that specifically labels protein thiol groups through covalent binding. This compound effectively interacts with protein amino and imidazole groups under neutral pH, making it suitable for diverse biological applications. It is widely used for the fluorescent labeling of proteins, nucleic acids, or other molecules containing one or more thiol groups, with an excitation/emission peak at 494/519 nm, facilitating visualization and analysis in various research settings.

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Specifications

Manufacturer Adooq Bioscience
CAS Number 2228857-33-6
Applications Fluorescein Derivative
Formula C27H19N3O7S
Molecular Weight 529.52
Synonyms 5-FITC-Maleimide
SMILES
O=C1OC2(C3=CC=C(NC(NCCN4C(C=CC4=O)=O)=S)C=C13)C5=CC=C(C=C5OC6=CC(O)=CC=C62)O

Storage & Solubility

In Vitro Solubility

Reported Solubility

No reported solubility data are available for this product.

Preparing Stock Solutions

Stock preparation is unavailable for this product molecular weight.

Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.

Storage

Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

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