HBC (HBC 530) is a synthetic RNA dye featuring a GFP fluorophore-like structure that employs a rigid electron acceptor coupled with a strong electron donor. Known for its low fluorescence background, HBC forms a stable complex with Pepper (RNA aptamer), resulting in bright fluorescence emission with a Kd of approximately 3.5 nM. Emission peaks can be tuned across a spectrum from cyan to red depending on the complex formed. This versatile dye is suitable for live-cell imaging applications of RNA, with an emission/excitation wavelength of 530/485 nm.
HBC (HBC 530) is a synthetic RNA dye featuring a GFP fluorophore-like structure that employs a rigid electron acceptor coupled with a strong electron donor. Known for its low fluorescence background, HBC forms a stable complex with Pepper (RNA aptamer), resulting in bright fluorescence emission with a Kd of approximately 3.5 nM. Emission peaks can be tuned across a spectrum from cyan to red depending on the complex formed. This versatile dye is suitable for live-cell imaging applications of RNA, with an emission/excitation wavelength of 530/485 nm.
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