Fig. 1 · Structure
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Hydroxyphenyl Fluorescein
ROS Probe
Hydroxyphenyl Fluorescein (HPF) functions as a stable fluorescent probe specifically targeting reactive oxygen species (ROS). This compound exhibits enhanced specificity and stability compared to traditional probes such as H2DCFDA. It generates strong green fluorescence upon reaction with intracellular peroxynitroso, making it suitable for studying oxidative stress. Hydroxyphenyl Fluorescein can be utilized in various applications, including fluorescence microscopy, high-throughput imaging, luciferase microplate assays, and flow cytometry. Excitation and emission wavelengths are 490 nm and 515 nm, respectively.
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| CAS Number | 359010-69-8 |
| Applications | ROS Probe |
| Formula | C26H16O6 |
| Molecular Weight | 424.40 |
| Synonyms | HPF; 3'-p-(Hydroxyphenyl) fluorescein |
| SMILES | OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1 |
Storage & Solubility
In Vitro Solubility
Reported Solubility
No reported solubility data are available for this product.
Preparing Stock Solutions
| Concentration | |
|---|---|
Values use four significant digits. Prepare to the final solution volume; this is not the volume of solvent to add.
Stock preparation is unavailable for this product molecular weight.
Calculated stock concentrations do not establish solvent solubility. Refer to the reported solubility data for experimental guidance.
Storage
Store powder at -20°C, keep desiccated.
In powder form, the chemical is stable for 36 months.
In solution, store at -20°C and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.