ONOO-LysopH is a Rhodamine-based near-infrared fluorescent dye specifically designed to target lysosomal pH and detect peroxynitrite (ONOO−). This probe exhibits a significant fluorescence enhancement at approximately 686 nm under acidic conditions, while showing a rapid NIR turn-on at around 678 nm in the presence of ONOO− at physiological pH. ONOO-LysopH is ideal for live cell imaging to monitor lysosomal pH fluctuations and to visualize endogenous or exogenous peroxynitrite levels, thereby providing valuable insights into cellular processes.
ONOO-LysopH is a Rhodamine-based near-infrared fluorescent dye specifically designed to target lysosomal pH and detect peroxynitrite (ONOO−). This probe exhibits a significant fluorescence enhancement at approximately 686 nm under acidic conditions, while showing a rapid NIR turn-on at around 678 nm in the presence of ONOO− at physiological pH. ONOO-LysopH is ideal for live cell imaging to monitor lysosomal pH fluctuations and to visualize endogenous or exogenous peroxynitrite levels, thereby providing valuable insights into cellular processes.
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