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Biochemical Assay Reagents collects small molecules used to prepare, run and calibrate biochemical and cell-based assays. Vitamins and cofactors such as niacin, riboflavin and biotin sit alongside sugars, sugar alcohols and amino acids such as L-arginine. Enzyme substrates and metal-ion chelators complete the range, including the calcium chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA). Three subcategories group related reagents, namely Carbohydrates & Glycobiology, Acyl-CoA & Cofactors for acyl-coenzyme A (acyl-CoA) compounds, and Chelators & Bifunctional Chelators. Most of these compounds act as substrates, cofactors, standards or buffer components rather than as inhibitors of a defined target.
Synonyms: 1-Methoxy PMS; 1-Methoxyphenazine methosulfate
Methoxy-PMS (1-Methoxy PMS), an active oxygen formation inducer, is stable electron-transport mediator between NAD(P)H and tetrazolium dyes.
Synonyms: β-DPN sodium; β-NAD sodium; β-Nicotinamide Adenine Dinucleotide sodium
NAD sodium is a crucial cofactor and homeostatic regulator that plays a significant role in cellular metabolism. It can be reduced to β-nicotinamide adenine dinucleotide (NADH) during oxidative reactions, facilitating ATP production within mitochondria. This compound is particularly relevant in research studying macrophage M2 polarization, non-alcoholic fatty liver disease, obesity, and glucose intolerance, making it a valuable reagent for investigations into metabolic syndrome and associated disorders.
Synonyms: Isopropyl β-D-thiogalactoside
IPTG (Isopropyl β-D-1-thiogalactopyranoside) acts as a molecular mimic of allolactose and serves as an inducer of the lac operon. This compound effectively triggers transcription in systems where gene expression is regulated by the lac operator, enabling controlled protein expression in biological research. IPTG is widely utilized in molecular biology and recombinant protein studies to regulate the activity of target genes within prokaryotic expression systems.
Thioglycolate sodium is an inflammation inducer that stimulates neutrophil and macrophage recruitment in vivo. This reagent is commonly utilized in research to induce peritonitis, allowing for the study of inflammatory responses and mechanisms in various biological contexts.
Synonyms: 1,2-Dimethylhydrazine dihydrochloride
N,N'-Dimethylhydrazine dihydrochloride is a potent tumor inducer targeting colon cancer pathways. This compound is widely used in rodent models to study the mechanisms of tumorigenesis and cancer progression. It serves as a valuable tool in cancer research, enabling the investigation of therapeutic approaches and the assessment of potential chemopreventive agents.
Synonyms: 2-O-beta-D-Glucopyranosyl-D-glucopyranose monohydrate
Sophorose monohydrate is a disaccharide that acts as a biochemical assay reagent, primarily distinguished by its role in microbial glycolipids. This compound exhibits significant biological activities, including antibacterial, antifungal, spermicidal, virucidal, and anticancer properties. In research applications, sophorose monohydrate has been identified as a potent inducer of cellulase gene expression in Trichoderma reesei fermentation, making it valuable for studies focused on enzyme production and biosurfactant development.
Synonyms: β-D-1-thiogalactopyranoside hemidioxane
IPTG hemidioxane, also known as β-D-1-thiogalactopyranoside hemidioxane, serves as a potent biochemical reagent primarily utilized as an inducer of the lac operon in molecular biology research. It effectively promotes the expression of proteins in bacterial systems, enhancing the study of gene function and protein production. This reagent is essential for assays requiring controlled gene expression and is widely used in recombinant protein technology.
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