Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Endogenous proteins maintain stable levels within cells under consistent environmental conditions due to a delicate balance between their production and degradation. However, this balance is disrupted when cells are examined outside their natural environment, leading to a significant reduction in protein synthesis and an increase in protein breakdown. To counteract this, a specialized mixture of enzyme inhibitors can be used to protect these proteins from degradation.

This mixture, known as a protease inhibitor cocktail, is applied to cell lysates or tissue extracts to preserve protein integrity. It works by blocking the action of proteases, which are enzymes that could potentially break down both phosphorylated and non-phosphorylated protein forms.

The protease inhibitor cocktail is specifically designed and verified for use with mammalian cells and tissues. It is a comprehensive solution targeting a variety of proteases, including serine, cysteine, and acid proteases, as well as aminopeptidases. Provided as a pre-made solution in DMSO, this cocktail offers a convenient option for enhancing protein stability in experimental setups.