Phosphatase Inhibitor Cocktail (2 Tubes, 100X)
For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.
In Stock
Background & Details
Protein phosphorylation is a crucial reversible posttranslational modification that alters protein shape, impacting their activity, localization, and interaction with other molecules. A significant proportion of cellular proteins, possibly around 30%, undergo phosphorylation.
In cells, the levels of proteins are kept constant due to a balance between their synthesis and breakdown, especially when environmental conditions do not fluctuate. However, crude cellular extracts are rich in native enzymes like phosphatases and proteases, which can degrade proteins or modify them, including the removal of phosphate groups. To circumvent this, one effective strategy is the application of specific inhibitors that target these degradative enzymes.
The efficacy of preserving phosphorylation states of proteins is proportional to the effectiveness of the inhibitors used. Our products include two distinct types of inhibitor solutions, each designed to robustly block phosphatase activity, thereby ensuring more complete preservation of your protein samples' phosphorylated forms.
Components
| Tube # | Ingredient | 100x Conc. | Target |
|---|---|---|---|
| A (Aqueous) | Sodium Fluoride | 100 mM | Acid phosphatases |
| Sodium Orthovanadate | 100 mM | Alkaline phosphatases, PTPs, ATPases | |
| Sodium Tartrate | 400 mM | Acid phosphatases | |
| Sodium Molybdate | 115 mM | Acid and phosphoprotein phosphatases | |
| Imidazole | 200 mM | Alkaline phosphatases | |
| B (DMSO) | (-)-p-Bromotetramisole oxalate | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 500 μM | Ser/Thr phosphatases | |
| Microcystin LR, Microcystis aeruginosa | 500 nM | PP1 and PP2A |
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Specifications
| Manufacturer | Adooq Bioscience |
|---|---|
| Applications | Phosphatase Inhibitor Cocktail |