Rhod-5N is a cell-impermeant calcium-binding fluorescent dye that features a BAPTA chelating group linked to a rhodamine fluorophore. This dye is particularly effective in detecting cation content in MOPS buffer, demonstrating a higher selectivity for Cd2+ over other common cations such as Na+, K+, Mg2+, Ca2+, and Zn2+, with the exception of Pb2+. Its sensitivity allows for detection at a limit of 3.1 μg/L, making it a valuable tool for research applications in calcium signaling and metal ion detection.
Rhod-5N is a cell-impermeant calcium-binding fluorescent dye that features a BAPTA chelating group linked to a rhodamine fluorophore. This dye is particularly effective in detecting cation content in MOPS buffer, demonstrating a higher selectivity for Cd2+ over other common cations such as Na+, K+, Mg2+, Ca2+, and Zn2+, with the exception of Pb2+. Its sensitivity allows for detection at a limit of 3.1 μg/L, making it a valuable tool for research applications in calcium signaling and metal ion detection.
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