2',7'-Difluorofluorescein is a pH-sensitive fluorescent probe with a pKa of approximately 4.7. It provides ratiometric pH analysis through altered fluorescence intensity upon excitation at 488 nm, facilitating quantitative pH measurements between 2 and 7, particularly in submicron aerosol particles. This compound is notable for its ability to undergo buffer-mediated excited-state proton transfer and maintain stable fluorescence characteristics in physiological pH ranges, making it suitable for applications in fluorescent imaging, calcium indicator systems, and as a fluorescent protein label. Additionally, it demonstrates resistance to photodegradation, ensuring reliability in experimental settings.
2',7'-Difluorofluorescein is a pH-sensitive fluorescent probe with a pKa of approximately 4.7. It provides ratiometric pH analysis through altered fluorescence intensity upon excitation at 488 nm, facilitating quantitative pH measurements between 2 and 7, particularly in submicron aerosol particles. This compound is notable for its ability to undergo buffer-mediated excited-state proton transfer and maintain stable fluorescence characteristics in physiological pH ranges, making it suitable for applications in fluorescent imaging, calcium indicator systems, and as a fluorescent protein label. Additionally, it demonstrates resistance to photodegradation, ensuring reliability in experimental settings.
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