5-CFDA-AM is a fluorescent probe designed to measure enzyme activity and assess cell membrane integrity. This cell-permeable esterase substrate enters cells efficiently and is hydrolyzed by intracellular esterases to form carboxyfluorescein, which exhibits enhanced retention due to its negative charge. Its quantitative fluorescence capacity makes it a valuable tool for various biological research applications, including studies on cellular processes and viability assessments.
5-CFDA-AM is a fluorescent probe designed to measure enzyme activity and assess cell membrane integrity. This cell-permeable esterase substrate enters cells efficiently and is hydrolyzed by intracellular esterases to form carboxyfluorescein, which exhibits enhanced retention due to its negative charge. Its quantitative fluorescence capacity makes it a valuable tool for various biological research applications, including studies on cellular processes and viability assessments.
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