SYTO9 is a green fluorescent nucleotide dye that selectively binds to nucleic acids, with excitation at 485 nm and emission at 498 nm for DNA and 501 nm for RNA. This dye exhibits the ability to penetrate intact cell membranes of viable bacteria, with increased permeability observed in compromised membranes. SYTO9 is commonly utilized in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology, enabling applications in bacterial viability assessment, cell imaging, and quantification of microbial populations.
SYTO9 is a green fluorescent nucleotide dye that selectively binds to nucleic acids, with excitation at 485 nm and emission at 498 nm for DNA and 501 nm for RNA. This dye exhibits the ability to penetrate intact cell membranes of viable bacteria, with increased permeability observed in compromised membranes. SYTO9 is commonly utilized in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology, enabling applications in bacterial viability assessment, cell imaging, and quantification of microbial populations.
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