Cell Culture and Assays

Cell culture and assay reagents support cell growth in vitro and the measurement of viability and proliferation. Serum-free media often add hormones such as insulin, hydrocortisone and liothyronine, the active thyroid hormone, together with L-glutamine as a nutrient. Enzymes such as trypsin and hyaluronidase help dissociate adherent cells and tissue fragments. Tetrazolium salts such as MTT and WST-8 report metabolic activity through the formation of a colored formazan. 5-Ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) label newly synthesized DNA in dividing cells. Calcein-AM and propidium iodide distinguish live from dead cells by esterase activity and membrane integrity.

2 Items

  • Resazurin sodium
    Cat. No.
    A44577

    Resazurin sodium

    Synonyms: Diazoresorcinol sodium

    Resazurin sodium is a non-toxic, stable redox indicator that targets bacterial activity through its membrane-permeable properties. It serves as a vital tool for assessing cell viability, proliferation, and toxicity in various cell types, including human, plant, animal, bacterial, and fungal cells. Upon reduction, Resazurin sodium transforms into Resorufin, a highly fluorescent compound, enabling sensitive detection and quantification of cellular processes under fluorescent microscopy or spectrophotometry.

    500 mg
    $20.00
    1 g
    $25.00
    All 3 sizes
  • Poly-L-lysine hydrochloride
    Cat. No.
    A47906

    Poly-L-lysine hydrochloride

    Poly-L-lysine hydrochloride is a cationic polymer that functions as a bacterial inhibitor by enhancing cell adhesion through electrostatic interactions with negatively charged cell membranes. It promotes liquid-liquid phase separation (LLPS) at low concentrations while inhibiting LLPS at elevated concentrations, making it a versatile tool for cell culture applications. This reagent is particularly useful in studies involving bacterial attachment mechanisms and cell substrate interactions.

    5 mg
    $25.00
    10 mg
    $40.00
    All 5 sizes

2 Items

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