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Stem cell reprogramming and differentiation reagents are small molecules that steer cell identity in culture. Chemical cocktails can replace or support transcription factors when somatic cells are reprogrammed into induced pluripotent stem cells (iPSCs). Frequent components include the histone deacetylase (HDAC) inhibitor valproic acid and the lysine-specific demethylase 1 (LSD1) inhibitor tranylcypromine. RepSox blocks the transforming growth factor beta (TGF-β) type I receptor and can replace the transcription factor Sox2. Inhibition of glycogen synthase kinase 3 (GSK-3) with CHIR-99021 and of MAP kinase kinase (MEK) with PD0325901 keeps mouse embryonic stem cells in the ground state. Differentiation protocols use retinoic acid, the Hedgehog pathway agonists SAG and purmorphamine, and IDE1 for definitive endoderm.
Purmorphamine is a 2,6,9-trisubstituted purine that promotes the differentiation of both human and mouse mesenchymal progenitor cells into osteoblasts.
Synonyms: Smoothened Agonist
SAG is a chlorobenzothiophene-containing compound which acts as a SMO agonist (EC50 = 3 nM) but inhibits hedgehog signaling at high concentrations (>1 uM).
Synonyms: Retinoic Acid; ATRA; Vitamin A Acid
Tretinoin is an endogenous agonist for retinoic acid receptors with IC50 value of 14 nM for RAR-alpha, RAR-beta and RAR-gamma receptors).
OAC1 enhances iPSC reprogramming efficiency by enhancing Oct4 and Nanog driven gene expression.
Synonyms: SR-1
StemRegenin 1 (SR1) is a cell-permeable purine derivative that acts as an antagonist of aryl hydrocarbon receptor and promotes the self-renewal of hematopoietic stem cells (HSCs).
Synonyms: Arotinoid acid; Ro 13-7410
TTNPB enhances reprogramming efficiency in chemically induced pluripotent stem cells (CiPSCs).
SAG (hydrochloride) is a potent Smo receptor agonist, and activates the Hedgehog signaling pathway, with a Kd of 59 nM.
IDE2 is a cell-permeable small molecule that promotes definitive endoderm formation in both mouse and human embryonic stem cells (ESCs) through activation of the TGF-β signaling pathway.
Synonyms: SR1 hydrochloride
Stemregenin 1 hydrochloride is a purine derivative that acts as an antagonist of the aryl hydrocarbon receptor (AhR). It has been shown to inhibit AhR signaling in CD34+ cells with an IC50 value of 127 nM. This compound is utilized in stem cell research to enhance the pluripotency and expansion of hematopoietic stem cells (HSC), providing valuable insights into stem cell biology and regenerative medicine.
Oct4 inducer-1 is a potent activator of the Oct4 transcription factor, significantly enhancing the expression of Oct4 and Nanog promoters. This compound promotes the formation of induced pluripotent stem cells (iPSCs) and improves the efficiency of cell reprogramming while reducing the overall reprogramming time. It is a valuable tool for research applications in stem cell biology and regenerative medicine.
SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist, with an EC50 of 3 nM and a Kd of 59 nM. This compound effectively activates the Hedgehog signaling pathway, providing a robust mechanism to counteract the inhibitory effects of Cyclopamine. SAG dihydrochloride is valuable for research in developmental biology, cancer biology, and therapeutic strategies targeting Hedgehog-related pathways.
