Enzyme Activity Probes & Substrates

Cat. No.

A19826

CAS No.

119171-73-2

ADHP

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

Cat. No.

A21938

CAS No.

6578-06-9

BCIP

Synonyms: BCIP p-toluidine salt; X-phosphate p-toluidine salt

BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

Cat. No.

A22538

CAS No.

94367-21-2

Suc-Leu-Leu-Val-Tyr-AMC

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.

Cat. No.

A22715

CAS No.

2070009-61-7

Z-Gly-Gly-Arg-AMC acetate

Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).

Cat. No.

A22810

CAS No.

211990-57-7

Ac-IETD-AFC

Ac-IETD-AFC is a fluorogenic substrate of caspase-8, caspase-3, caspase-10, and granzyme B.

Cat. No.

A22829

CAS No.

500799-61-1

Abz-FR-K(Dnp)-P-OH

Abz-FR-K(Dnp)-P-OH is an angiotensin I-converting enzyme (ACE) substrate and an internally quenched fluorogenic substrate for real time fluorescent assay.

Cat. No.

A22858

CAS No.

65147-09-3

Boc-Leu-Gly-Arg-AMC

Boc-Leu-Gly-Arg-AMC is a fluorogenic AMC substrate for the convertases. Boc-Leu-Gly-Arg-AMC can be used in enzymatic assays.

Cat. No.

A22938

CAS No.

72252-90-5

MeOSuc-AAPV-AMC

MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).

Cat. No.

A22998

CAS No.

323577-36-2

Mca-(ala7,lys(dnp)9)-bradykinin

Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage.

Cat. No.

A23068

CAS No.

88467-45-2

Suc-AAPF-AMC

Synonyms: Suc-Ala-Ala-Pro-Phe-AMC

Suc-AAPF-AMC is an α-chymotrypsin fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Cat. No.

A23180

CAS No.

136132-77-9

Boc-Arg-Val-Arg-Arg-AMC hydrochloride

Synonyms: Boc-RVRR-AMC

Boc-Arg-Val-Arg-Arg-AMC hydrochloride (Boc-RVRR-AMC) is a synthetic fluorogenic substrate that is efficiently cleaved by furin.

Cat. No.

A23549

CAS No.

71973-79-0

Suc-Ala-Ala-Phe-AMC

Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays.

Cat. No.

A24667

CAS No.

150956-92-6

MMP-1/MMP-9 Substrate, Fluorogenic

Synonyms: Dnp-P-Cha-G-Cys(Me)-HA-K(Nma)-NH2

MMP-1/MMP-9 Substrate, Fluorogenic is a highly sensitive substrate specifically designed for the detection of matrix metalloproteinases MMP-1 and MMP-9. Upon cleavage by these enzymes, it emits fluorescence with excitation and emission wavelengths at 340 nm and 440 nm, respectively. This substrate is ideal for research applications involving extracellular matrix remodeling, cancer metastasis studies, and the assessment of tissue remodeling processes.

Cat. No.

A25173

CAS No.

189275-74-9

Ac-Trp-Glu-His-Asp-AMC

Synonyms: AC-WEHD-AMC

Ac-Trp-Glu-His-Asp-AMC is a specific fluorogenic substrate designed for caspase-1 detection and activity measurement. This compound exhibits high sensitivity and selectivity for caspase-1, enabling the quantitative assessment of apoptotic processes and inflammatory pathways. Its application in fluorescence assays facilitates the study of caspase-1's role in various biological contexts, including immune responses and cell death mechanisms.

Cat. No.

A25212

CAS No.

210344-99-3

Ac-WEHD-AFC

Ac-WEHD-AFC is a fluorogenic substrate specifically designed for the detection of caspase-1 activity. This compound enables the quantitative measurement of caspase-1 enzymatic function, making it a valuable tool in studies related to tumorigenesis and inflammatory processes. Its sensitive fluorescence readout facilitates the assessment of caspase-1 involvement in various biological systems.

Cat. No.

A25326

CAS No.

189696-01-3

Mca-YVADAP-Lys(Dnp)-OH

Mca-YVADAP-Lys(Dnp)-OH is a fluorogenic substrate designed for the characterization of caspase-1 and angiotensin-converting enzyme 2 (ACE2) activity. This compound enables the detection of enzymatic activity through fluorescence, making it valuable for studies focused on apoptosis and cardiovascular signaling pathways. It is suitable for use in various assays aimed at exploring the roles of these enzymes in cellular processes and disease states.

Cat. No.

A25446

CAS No.

210345-01-0

Ac-VEID-AFC

Ac-VEID-AFC is a fluorogenic substrate specifically designed for the detection of caspase-6 activity. Its cleavage by caspase-6 produces a fluorescent signal, making it a valuable tool for assessing apoptotic processes and related signaling pathways in various biological systems. This substrate is widely utilized in research applications focused on cell death, inflammation, and neurodegenerative diseases.

Cat. No.

A25451

CAS No.

919515-51-8

Ac-VRPR-AMC

Ac-VRPR-AMC is a fluorogenic substrate specifically designed for the detection of metacaspase activity. This compound is utilized in biochemical assays to measure metacaspase activity, making it a valuable tool for research in apoptosis and programmed cell death. Its fluorescence properties enable sensitive detection, facilitating studies on metacaspase function in various biological systems.

Cat. No.

A33612

CAS No.

168202-46-8

Abl Cytosolic Substrate

Synonyms: Abltide

Abl Cytosolic Substrate serves as an active substrate for the Abelson tyrosine kinase (Abl). This substrate is vital for studying the enzymatic activity of Abl, a truncated form of the v-Abl protein associated with the Gag-Abl fusion protein in Abelson murine leukemia virus. It is primarily utilized in research applications focusing on signal transduction and oncogenic processes mediated by tyrosine phosphorylation.

Cat. No.

A35115

CAS No.

172615-51-9

IGF1Rtide

IGF1Rtide is a specific substrate for Ret proto-oncogene kinase (RET) that facilitates the assessment of RET kinase activity in various biochemical assays. This peptide is instrumental in evaluating RET signaling pathways and investigating the role of RET in cancer biology and cellular signaling processes. Its use in kinase assays aids in the development of RET-targeted therapies and drug discovery efforts.

Cat. No.

A36181

CAS No.

3368-04-5

4-Methylumbelliferyl phosphate

Synonyms: 4-MUP; MUP

4-Methylumbelliferyl phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Upon dephosphorylation, it produces a fluorescent product, enabling sensitive detection in enzyme activity assays. This reagent is widely utilized in research applications involving phosphatase activity, enzymatic kinetics, and biological assays requiring fluorescent readout. Additionally, it serves as a nerve agent simulant in various experimental contexts.

Cat. No.

A36374

CAS No.

123359-43-3

Thymolphthalein monophosphate disodium hydrate

Thymolphthalein monophosphate disodium hydrate serves as a chromogenic substrate for the detection of acid and alkaline phosphatases. Upon enzymatic hydrolysis, it releases thymolphthalein, which increases the medium's pH, facilitating colorimetric detection and cessation of hydrolysis. This reagent is particularly valuable for the specific identification of prostatic phosphatase in serum samples, making it an essential tool in biochemical research and clinical diagnostics.

Cat. No.

A39795

CAS No.

88899-22-3

Bz-FVR-AMC

Bz-FVR-AMC is a fluorogenic substrate specifically designed for the assay of procathepsin activity. With a kcat/Km value of 1070 mM^-1s^-1, it provides a robust tool for measuring cathepsin enzyme activity in various biological samples. Bz-FVR-AMC is particularly useful for studying proteolytic processes and understanding cysteine protease functions in both physiological and pathological contexts. However, at high concentrations, this substrate may exhibit inhibitory effects.

Cat. No.

A39833

CAS No.

839730-93-7

Cathepsin D and E FRET Substrate

Cathepsin D and E FRET Substrate is a fluorogenic substrate specifically designed for the selective detection of cathepsins D and E, avoiding interaction with cathepsins B, H, and L. Cleavage occurs at the Phe-Phe amide bond, releasing a fluorescent signal that can be quantitatively measured. This substrate is ideal for routine assays and mechanistic studies, facilitating the investigation of cathepsin activity in various biological contexts.

Cat. No.

A39881

CAS No.

1135364-14-5

Z-Nle-Lys-Arg-AMC

Z-Nle-Lys-Arg-AMC is a fluorogenic peptide substrate designed to specifically detect cathepsin B activity across a wide pH range. This substrate undergoes cleavage by cathepsin B, resulting in fluorescence which can be quantitatively measured. It is particularly valuable in studies related to protease activity, cell signaling, and understanding pathological processes where cathepsin B is involved.