Buffers, Detergents & Protease Inhibitors

Buffers, detergents and protease inhibitors are basic reagents for preparing, lysing and stabilizing biological samples. Zwitterionic Good's buffers such as HEPES, MOPS, PIPES and MES hold pH steady in the physiological range, while Tris serves electrophoresis and general use. Detergents such as CHAPS, polysorbate 20 and sodium dodecyl sulfate (SDS) solubilize membranes and proteins. Dithiothreitol (DTT) keeps thiol groups reduced, and ethylenediaminetetraacetic acid (EDTA) chelates divalent metal ions. Protease inhibitors such as leupeptin, aprotinin, pepstatin A and E-64, with the phosphatase inhibitor sodium orthovanadate, protect proteins in cell lysates.

Items 76-100 of 105

  • TAPSO
    Cat. No.
    A88486

    TAPSO

    TAPSO is a biological buffer that stabilizes pH in the range of 7-8, making it suitable for a variety of biochemical applications. With a pKa value between 7.5 and 9.0, TAPSO effectively maintains physiological pH levels in laboratory environments. It contains Tris groups and demonstrates reactivity with zwitterionic glycine peptides, facilitating studies in protein chemistry and enzymatic reactions. This reagent is essential for experiments requiring precise pH control.

    10 g
    $20.00
    25 g
    $25.00
    All 3 sizes
  • BES sodium
    Cat. No.
    A89116

    BES sodium

    BES sodium is a biochemical buffer known for its effective pH range of 6.4 to 7.8, with a pKa value of 7.15 at 20 °C. This secondary standard buffer is particularly useful in diagnostic testing applications, providing stability in biochemical assays. Its ability to maintain pH under various conditions makes it an essential reagent in laboratory settings.

    10 g
    $20.00
    100 g
    $25.00
    All 3 sizes
  • MOPSO sodium
    Cat. No.
    A89127

    MOPSO sodium

    MOPSO sodium is a zwitterionic buffer primarily used to maintain stable pH conditions in biological and biochemical applications. Its unique properties allow it to minimize ionic strength fluctuations while offering high solubility, making it suitable for the formulation of buffer solutions in various laboratory settings. MOPSO sodium is ideal for applications in enzyme assays, protein purification, and molecular biology techniques requiring precise pH control.

    250 g
    $30.00
    500 g
    $55.00
    All 2 sizes
  • PIPES sesquisodium
    Cat. No.
    A89171

    PIPES sesquisodium

    PIPES sesquisodium is an organic pH buffer that stabilizes the acid-base balance within a range of 6.1 to 7.5. It is primarily utilized in the preparation of marine and mammalian tissues for electron microscopy, facilitating detailed structural analysis. Its effectiveness in maintaining steady pH conditions makes it a valuable reagent for various biological and biochemical research applications.

    5 g
    $20.00
    10 g
    $25.00
    All 5 sizes
  • EDTA tetrasodium tetrahydrate
    Cat. No.
    A89279

    EDTA tetrasodium tetrahydrate

    Synonyms: Ethylenediaminetetraacetic acid tetrasodium tetrahydrate

    EDTA tetrasodium tetrahydrate is a potent metal chelator that effectively binds divalent and trivalent metal cations, including calcium. This compound exhibits anticoagulant properties and demonstrates anti-hypercalcemic activity, making it valuable in various biological research applications. Additionally, EDTA tetrasodium tetrahydrate reduces metal ion-catalyzed oxidative damage to proteins and helps maintain a reducing environment during protein purification, while also minimizing the formation of disulfide bonds.

    1 mg
    $20.00
    500 g
    $30.00
    All 2 sizes
  • DIPSO
    Cat. No.
    A89310

    DIPSO

    DIPSO is a zwitterionic buffer that effectively maintains a stable pH range from 7.0 to 8.2, making it suitable for various biological applications. It has been shown to disrupt meiotic regulation in mouse oocytes, providing valuable insights into cell cycle control. Additionally, DIPSO exhibits surfactant properties at a concentration of 10 mM, enhancing its utility in biochemical assays and cell culture techniques.

    10 g
    $25.00
    100 g
    $65.00
    All 2 sizes
  • MES hemisodium
    Cat. No.
    A89401

    MES hemisodium

    Synonyms: 2-Morpholinoethanesulphonic acid hemisodium

    MES hemisodium (2-Morpholinoethanesulphonic acid) functions as an amphoteric ion buffer, with an effective buffering range of pH 5.5 to 7.0. This Good's buffer is commonly utilized in biochemistry and molecular biology applications, including cell culture, enzyme activity assays, electrophoresis, and protein research. Its stability and compatibility with various biological systems make it an essential reagent in laboratory experiments.

    25 g
    $20.00
    50 g
    $25.00
    All 3 sizes
  • Ethylenediaminetetraacetic acid diammonium
    Cat. No.
    A89676

    Ethylenediaminetetraacetic acid diammonium

    Synonyms: EDTA diammonium

    Ethylenediaminetetraacetic acid diammonium is a chelating agent with strong metal ion binding properties. It is employed in biochemical assays to sequester trace metals, thereby enhancing product quality and stability in various industries, including food and pharmaceuticals. Additionally, this compound serves as an anticoagulant in blood collection tubes and functions as a clarifying agent in cosmetic and personal care formulations.

    Out of stock

    All 3 sizes
  • EDTA dihydrate tripotassium
    Cat. No.
    A90075

    EDTA dihydrate tripotassium

    Synonyms: Ethylenediaminetetraacetic acid dihydrate tripotassium

    EDTA dihydrate tripotassium is a biochemical assay reagent that acts as a versatile chelating agent, effectively binding metal ions. It is primarily utilized in life science research to eliminate interference from metal ions in biochemical assays and to stabilize enzymatic reactions. This reagent facilitates the study of various biological processes by maintaining optimal conditions and enhancing the accuracy of experimental results.

    25 g
    $20.00
    50 g
    $35.00
    All 2 sizes
  • ADA sodium
    Cat. No.
    A90274

    ADA sodium

    ADA sodium is an effective buffer used to maintain pH stability in biological and chemical systems. This compound is crucial for experiments requiring a consistent pH environment, thereby enhancing the accuracy and reproducibility of results in various biochemical assays. Its application spans across cell culture, enzymatic reactions, and other research areas where pH influences biological activity.

    Out of stock

    All 3 sizes
  • DIPSO sodium
    Cat. No.
    A90279

    DIPSO sodium

    DIPSO sodium is a zwitterionic buffer reagent that effectively maintains pH stability in biochemical research applications. It is particularly useful in biochemical assays and chromatographic techniques, facilitating optimal conditions for various enzymatic reactions and protein studies. Its unique buffering capacity makes it suitable for a wide range of experimental conditions, enhancing the reliability of research outcomes.

    Out of stock

    All 3 sizes
  • CAPSO sodium
    Cat. No.
    A90332

    CAPSO sodium

    CAPSO sodium is a zwitterionic buffer primarily utilized for stabilizing pH in biological research. It has an effective pH range of 8.9 to 10.3, making it suitable for various biochemical applications. This reagent is commonly employed in enzyme assays, protein purification, and other experimental protocols requiring minimal ionic interference.

    25 g
    $20.00
    50 g
    $30.00
    All 2 sizes
  • EDTA diammonium salt hydrate, 97%
    Cat. No.
    A90859

    EDTA diammonium salt hydrate, 97%

    EDTA diammonium salt hydrate is a chelating agent derived from ethylenediaminetetraacetic acid (EDTA) known for its ability to form stable complexes with a wide range of metal ions. It exhibits effective metal ion binding properties, making it valuable in biological and chemical research. This reagent is commonly utilized in applications such as metal ion extraction, stabilization of enzymes, and removal of heavy metals from solutions.

    Out of stock

    All 3 sizes
  • Tris phosphate dibasic
    Cat. No.
    A91078

    Tris phosphate dibasic

    Tris phosphate dibasic is a biochemical assay reagent that serves as a buffer component to maintain pH stability in various biochemical experiments. Its primary mechanism involves acting as a hydroxyl ion donor, which supports enzyme activity and stability during assays. Tris phosphate dibasic is commonly utilized in molecular biology applications, including nucleic acid purification and protein assays. It is essential for facilitating reactions that require a controlled environment to ensure accurate and reproducible results.

    Out of stock

    All 3 sizes
  • MOPS hemisodium salt, 98%
    Cat. No.
    A91185

    MOPS hemisodium salt, 98%

    MOPS hemisodium salt is a buffering agent that effectively maintains physiological pH in biochemical and molecular biology experiments. It is particularly useful for applications involving the stabilization of nucleic acids and proteins, enabling reproducibility in assays. With its ability to sustain a consistent pH environment, MOPS hemisodium salt is essential for a variety of laboratory protocols requiring stringent pH conditions.

    10 g
    $25.00
    All 1 sizes
  • Ethylenediaminetetraacetic acid dilithium
    Cat. No.
    A91364

    Ethylenediaminetetraacetic acid dilithium

    Synonyms: EDTA dilithium

    Ethylenediaminetetraacetic acid dilithium salt (EDTA-DLi) acts as a powerful chelating agent, effectively binding metal ions in biochemical assays. This reagent is widely utilized in research applications aimed at studying metal ion interactions, enzyme activity modulation, and protein purification processes. Its ability to stabilize samples and inhibit metal-catalyzed reactions makes it essential for various life science investigations.

    Out of stock

    All 3 sizes
  • IPTG hemidioxane
    Cat. No.
    A91745

    IPTG hemidioxane

    Synonyms: β-D-1-thiogalactopyranoside hemidioxane

    IPTG hemidioxane, also known as β-D-1-thiogalactopyranoside hemidioxane, serves as a potent biochemical reagent primarily utilized as an inducer of the lac operon in molecular biology research. It effectively promotes the expression of proteins in bacterial systems, enhancing the study of gene function and protein production. This reagent is essential for assays requiring controlled gene expression and is widely used in recombinant protein technology.

    Out of stock

    All 3 sizes
  • TAPSO sodium
    Cat. No.
    A91976

    TAPSO sodium

    TAPSO sodium is a biochemical assay reagent that acts as a buffering agent in biological research. It stabilizes pH in various reaction environments, enhancing the reliability of experimental results. TAPSO sodium is commonly utilized in enzymatic assays and protein studies, facilitating accurate measurements and observations in life science investigations.

    Out of stock

    All 3 sizes
  • EDTA magnesium disodium,99%
    Cat. No.
    A92180

    EDTA magnesium disodium,99%

    EDTA magnesium disodium, 99% is a potent chelating agent that effectively binds metal ions such as calcium, magnesium, and iron. Its primary mechanism involves forming stable complexes with these ions, facilitating their removal from solution. This reagent is widely utilized in biochemical and molecular biology research to control metal ion concentrations and study their effects on biological systems.

    Out of stock

    All 3 sizes
  • TAPS sodium
    Cat. No.
    A92309

    TAPS sodium

    Synonyms: TAPS Na

    TAPS sodium is a biological buffer targeting optimal pH stability in biochemical applications. With a pKa of 8.1, it maintains the structural integrity of proteins, such as lysozyme, and prevents thermal denaturation at elevated temperatures. This compound is essential for research involving connexin channel activity and related membrane protein studies.

    Out of stock

    All 3 sizes
  • Ethylenediaminetetraacetic acid trisodium hydrate
    Cat. No.
    A92650

    Ethylenediaminetetraacetic acid trisodium hydrate

    Synonyms: EDTA trisodium hydrate

    Ethylenediaminetetraacetic acid trisodium hydrate functions primarily as a chelating agent, targeting metal ions with high affinity. It is widely utilized in biochemical assays, where it effectively binds trace metal contaminants that could compromise the quality of samples or affect experimental outcomes. Additionally, this compound serves as an anticoagulant in blood collection systems and acts as a stabilizing agent in cosmetic formulations, making it valuable in both pharmaceutical and regulatory applications.

    Out of stock

    All 3 sizes
  • Tris hemisulfate,98%
    Cat. No.
    A92760

    Tris hemisulfate,98%

    Tris hemisulfate is a biochemical assay reagent that functions primarily as a buffering agent, maintaining pH stability in biological and chemical systems. It is crucial for various laboratory applications including protein purification, enzyme assays, and nucleic acid studies. This compound facilitates optimal conditions for biochemical reactions, ensuring accurate and reproducible results in research settings.

    Out of stock

    All 3 sizes
  • EDTA dipotassium magnesium
    Cat. No.
    A92785

    EDTA dipotassium magnesium

    Synonyms: Ethylenediaminetetraacetic acid dipotassium magnesium

    EDTA dipotassium magnesium is a chelating agent that effectively binds divalent cations, particularly magnesium, in biochemical assays. This compound is widely utilized in life science research for its ability to stabilize metal ion concentrations and facilitate enzyme activities that require magnesium as a cofactor. Its applications include the study of enzymatic reactions, metal ion interactions, and various biochemical pathways.

    5 g
    $45.00
    10 g
    $70.00
    All 3 sizes
  • Glycylglycine hydrochloride hydrate
    Cat. No.
    A93499

    Glycylglycine hydrochloride hydrate

    Synonyms: Diglycine hydrochloride hydrate; Gly-Gly (HCl H2O)

    Glycylglycine hydrochloride hydrate is an iNOS inhibitor that acts as a non-selective dipeptidase substrate. This compound is capable of passive diffusion across cell membranes, where it is hydrolyzed to glycine, playing a role in energy metabolism and antioxidant activities. Glycylglycine hydrochloride hydrate promotes the proliferation of spermatogonial stem cells and mitigates astrocyte overactivation while decreasing nitric oxide release. Additionally, it upregulates the expression of neurotrophic factors, aiding in nerve myelin repair. This reagent is suitable for research into male reproductive biology and the neuroprotective mechanisms in neurodegenerative diseases.

    5 mg
    $35.00
    10 mg
    $60.00
    All 5 sizes
  • POPSO sodium
    Cat. No.
    A93585

    POPSO sodium

    POPSO sodium is a zwitterionic buffer commonly employed in biochemical assays to maintain stable pH conditions. Its unique structure allows for effective buffering across a range of pH levels, making it suitable for various biological applications. POPSO sodium is particularly useful in protein purification, enzyme activity assays, and electrophoresis, ensuring reliable results in research and analytical procedures.

    Out of stock

    All 3 sizes

Items 76-100 of 105

Added to your cart

View Cart Checkout

    Loading...