Buffers, Detergents & Protease Inhibitors

Cat. No.

A86693

CAS No.

1135-40-6

CAPS

Synonyms: N-Cyclohexyl-3-aminopropanesulfonic acid

CAPS, or cyclohexylaminopropane sulfonic acid, acts as an effective surfactant. It serves as a biological buffer at a concentration of 0.05 M and pH 11, making it suitable for applications such as dialysis and protein purification. Its unique properties facilitate improved solubility and stability in various biological systems, enhancing experimental outcomes in research.

Cat. No.

A86772

CAS No.

1266615-59-1

MES hydrate

Synonyms: 2-Morpholinoethanesulphonic acid hydrate

MES hydrate (2-Morpholinoethanesulphonic acid hydrate) serves as an amphoteric ion buffer, providing optimal buffering capacity in the pH range of 5.5 to 7.0. Widely utilized in biochemistry and molecular biology, MES hydrate is ideal for applications such as cell culture, enzyme assays, electrophoresis, and protein characterization. Its stability and efficacy make it a valuable reagent for various experimental settings.

Cat. No.

A86788

CAS No.

71119-23-8

MES sodium salt

Synonyms: 2-Morpholinoethanesulphonic acid sodium salt

MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with optimal buffering capacity within the pH range of 5.5 to 7.0. This Good's buffer is commonly utilized in biochemical and molecular biology applications, including cell culture, enzyme activity assays, electrophoresis, and protein analysis. Its reliable pH stability makes it a valuable choice for various laboratory experiments requiring precise pH conditions.

Cat. No.

A86927

CAS No.

13368-13-3

EGTA tetrasodium

EGTA tetrasodium is a specialized calcium ion chelator with a primary mechanism of chelating Ca2+ ions. It exhibits a low apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4), demonstrating high specificity for calcium over magnesium (Kd for Mg2+ is 1-10 mM). This compound is utilized in research applications focusing on calcium-dependent cellular processes, including the inhibition of substrate adherence in inflammatory macrophages.

Cat. No.

A86965

CAS No.

150-25-4

Bicine

Bicine is a biological buffer that serves to maintain pH stability in physiological research. As a derivative of glycine, Bicine enhances the sensitivity of guanosine enzyme histochemical detection, making it a valuable tool in biochemical assays and analytical studies. Its capability to stabilize pH under various conditions makes it suitable for applications in enzyme studies and other biochemical processes.

Cat. No.

A87158

CAS No.

76836-02-7

PIPES disodium

Synonyms: 1,4-Piperazinediethanesulfonic acid disodium

PIPES disodium is a buffering agent known for its effective buffering capacity in a physiological pH range. It stabilizes the pH of biological and biochemical systems, making it suitable for a variety of laboratory applications, including enzyme assays, cell culture, and electrophoresis. The compound is especially useful for maintaining the stability of biological samples and reagents during experimental procedures.

Cat. No.

A87162

CAS No.

124763-51-5

Bis-Tris hydrochloride

Bis-Tris hydrochloride is a zwitterionic buffer primarily used to maintain a stable pH in biochemical applications. It effectively supports various research applications, including gel electrophoresis, cell culture, and bioprocessing, due to its low absorption in the UV range and its capacity to stabilize biological samples. This buffer is particularly advantageous for experiments requiring precise pH control without interfering with biological processes.

Cat. No.

A87177

CAS No.

71119-22-7

MOPS sodium salt

MOPS sodium salt is a buffering agent primarily utilized to maintain physiological pH levels in mammalian cell culture media. This compound is essential for optimizing conditions in various biological assays, ensuring consistency in experimental results. Additionally, MOPS sodium salt significantly enhances the thermal stability of bovine serum albumin (BSA), making it valuable for studies involving protein stability and activity.

Cat. No.

A87214

CAS No.

7365-44-8

TES

TES (N-Tris[hydroxymethyl]methyl-2-aminoethanesulfonic acid) serves as a highly effective hydrogen ion buffering agent, suitable for maintaining physiological pH levels in various biological applications. It exhibits an appropriate dissociation constant, low metal-binding capacity, and notable water solubility, making it ideal for mammalian cell culture studies and viral research. Researchers utilize TES to enhance the accuracy of experimental conditions where pH stability is critical.

Cat. No.

A87278

CAS No.

39946-25-3

MES potassium

Synonyms: 2-Morpholinoethanesulphonic acid potassium

MES potassium, or 2-Morpholinoethanesulphonic acid potassium, acts as an amphoteric ion buffer effective in the pH range of 5.5 to 7.0. This Good's buffer is commonly employed in various biochemical and molecular biology applications, including cell culture, enzyme activity assays, electrophoresis, and protein characterization studies. Its stable buffering capacity makes it essential for experiments requiring precise pH control.

Cat. No.

A87364

CAS No.

7365-82-4

ACES

Synonyms: N-(2-Acetamido)-2-aminoethanesulfonic acid

ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid) functions as a zwitterionic buffer, stabilizing pH levels in biochemical assays and biological research. It exhibits optimal buffering capacity within the pH range of 6.8 to 7.2, making it suitable for applications requiring precise pH conditions, such as enzyme assays and cell culture studies. ACES is particularly beneficial in experiments involving sensitive biological systems where maintaining pH is critical for accurate results.

Cat. No.

A87382

CAS No.

103404-87-1

HEPES hemisodium salt, 99%(T)

HEPES hemisodium salt, 99% (T) serves as an effective buffering agent commonly employed in biological research. Its primary mechanism involves maintaining a stable pH in various laboratory applications, particularly in cell culture and biochemical assays. This reagent is essential for experiments requiring precise pH control, contributing to the reliability and reproducibility of results in studies involving proteins, nucleic acids, and enzymatic reactions.

Cat. No.

A87414

CAS No.

68399-77-9

MOPSO

MOPSO is a zwitterionic buffer that provides effective pH stabilization in the range of 6.2 to 7.6. It is commonly used in biological applications, including as a buffer component in charcoal yeast extract medium, facilitating optimal growth conditions for various microorganisms. MOPSO is particularly valuable in studies requiring precise control of pH to ensure reproducible results.

Cat. No.

A87571

CAS No.

10191-18-1

BES

BES (N,N-bis(2-hydroxyethyl)2-aminoethanesulfonic acid) is a secondary standard biochemical buffer designed to maintain physiological pH levels, with an effective range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is commonly employed in diagnostic testing applications, facilitating accurate biochemical analyses by stabilizing pH. Its buffering capacity makes it a valuable tool in various laboratory settings that require precise pH control.

Cat. No.

A87614

CAS No.

85715-60-2

EDTA hydrate sodium

Synonyms: Ethylenediaminetetraacetic acid hydrate sodium

EDTA hydrate sodium, a metal chelator, effectively binds to bivalent and trivalent metal cations, including calcium. This compound exhibits diverse biological activities such as antibacterial, anti-inflammatory, antioxidant, and anticoagulant properties. It is instrumental in reducing metal ion-catalyzed oxidative damage to proteins, facilitating a reducing environment essential for protein purification. Additionally, EDTA hydrate sodium shows potential in alleviating liver fibrosis and is utilized in research pertaining to coronary artery disease and neurological disorders.

Cat. No.

A87726

CAS No.

331717-45-4

CHAPS hydrate

CHAPS hydrate is a zwitterionic detergent derived from cholic acid, functioning primarily as a micelle-forming agent. It offers weak cationic or nonionic surfactant properties, undergoing micellization to form hydrophilic aggregates that are sensitive to temperature changes. CHAPS hydrate is instrumental in stabilizing mononucleosomes across various ionic strengths, facilitating histone core sliding along DNA, and solubilizing Tamm‑Horsfall protein to improve urinary exosome isolation. This reagent is widely employed in the recovery of native folded fusion proteins, enhancing GST fusion protein binding capacity, and maintaining protein activity during membrane protein research, although it is ineffective for refolding proteins denatured by strong agents.

Cat. No.

A87969

CAS No.

73463-39-5

CAPSO

CAPSO, a zwitterionic buffer, is designed to maintain stable pH levels in the range of 8.9 to 10.3. It is particularly effective in biological applications where maintaining a consistent pH is critical for enzymatic reactions and protein stability. CAPSO is commonly used in biochemical assays, protein purification, and electrophoresis, making it an essential reagent for researchers in life sciences.

Cat. No.

A87999

CAS No.

68189-43-5

POPSO

POPSO is a zwitterionic buffer that serves to regulate osmolality in biochemical assays. It exhibits significant inhibition of anion uniport, particularly chloride uniport, with an IC50 value of 24 mM. Additionally, POPSO has been shown to enhance copper uptake and toxicity in algal systems while disrupting mitochondrial inner membrane integrity. The optimal working pH range for POPSO sesquisodium salt is 7.2 to 8.5, making it suitable for various research applications involving cellular and biochemical studies.

Cat. No.

A88145

CAS No.

70331-82-7

TES sodium

TES sodium is a potent hydrogen ion buffer with a favorable dissociation constant, low metal-binding capacity, and high solubility in water. It is particularly useful for maintaining pH stability in biological systems. TES sodium is commonly employed in the research of mammalian cell culture and virology, providing reliable conditions for experimental assays.

Cat. No.

A88185

CAS No.

10010-67-0

PIPES monosodium

Synonyms: 1,4-Piperazinediethanesulfonic acid monosodium

PIPES monosodium (1,4-Piperazinediethanesulfonic acid) serves as a versatile pH buffer, effectively stabilizing the pH of biological and chemical systems. This reagent can be combined with its disodium counterpart, allowing for precise control over pH and ionic strength by adjusting the ratios of the two forms. It is widely used in biochemical and molecular biology applications, particularly for enzyme reactions and cellular studies where pH regulation is critical.

Cat. No.

A88193

CAS No.

72200-76-1

TRIS maleate

TRIS maleate is a reliable buffering agent that maintains stable pH levels in biological experiments. It is commonly utilized in growth media, tissue preparations, and as a diluent or washing buffer, facilitating optimal conditions for various biochemical assays. Its capacity to enhance the solubility and stability of biomolecules makes it a valuable reagent in molecular biology and biochemistry research applications.

Cat. No.

A88204

CAS No.

108321-27-3

PIPES dipotassium

PIPES dipotassium is a zwitterionic buffer commonly used in biochemical and molecular biology applications. It effectively maintains stable pH levels in biological systems, particularly within the pH range of 6.1 to 7.5. PIPES dipotassium is ideal for applications such as protein purification, enzyme assays, and cell culture, ensuring optimal conditions for various biochemical reactions.

Cat. No.

A88272

CAS No.

161308-36-7

HEPBS

HEPBS is a zwitterionic buffering agent that operates within a physiological pH range of 7.6 to 9.0, making it suitable for use in various biochemical assays. Its chemical properties allow for optimal cell culture conditions, facilitating accurate experimental outcomes in biological research. HEPBS is particularly useful in studies requiring stable buffering to maintain consistent pH levels throughout the experimental process.

Cat. No.

A88321

CAS No.

41689-31-0

ADA disodium

ADA disodium is a buffer salt derived from adenosine deaminase, primarily targeting adenine metabolism. It is crucial for biochemical research, particularly in the formulation of enzyme buffers and optimizing enzymatic reactions. Its utility extends to assays that require stable pH conditions, facilitating accurate measurements in various biochemical and molecular biology applications.

Cat. No.

A88407

CAS No.

26239-55-4

ADA

Adenosine deaminase (ADA) is a biological buffer that plays a crucial role in maintaining pH stability in various biochemical applications. It can also act as a chelator, interacting with metal ions to enhance their bioavailability. ADA is widely utilized in the study of enzymatic reactions and microbial growth, making it essential for numerous laboratory protocols in life science research.