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Buffers, detergents and protease inhibitors are basic reagents for preparing, lysing and stabilizing biological samples. Zwitterionic Good's buffers such as HEPES, MOPS, PIPES and MES hold pH steady in the physiological range, while Tris serves electrophoresis and general use. Detergents such as CHAPS, polysorbate 20 and sodium dodecyl sulfate (SDS) solubilize membranes and proteins. Dithiothreitol (DTT) keeps thiol groups reduced, and ethylenediaminetetraacetic acid (EDTA) chelates divalent metal ions. Protease inhibitors such as leupeptin, aprotinin, pepstatin A and E-64, with the phosphatase inhibitor sodium orthovanadate, protect proteins in cell lysates.
POPSO sesquisodium is a zwitterionic buffer known for its water solubility. It is widely utilized in biological and biochemical research to stabilize the pH of solutions, particularly within the physiological range of 7.0 to 8.5. This property makes it ideal for various applications, including enzyme assays and cell culture studies, where maintaining consistent pH is critical for optimal performance and accurate results.
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Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride
TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that effectively reduces protein disulfide bonds without disrupting thiol-targeted processes. Its high stability and rapid reduction capability make TCEP hydrochloride an ideal choice for various biochemical applications. It is widely utilized in protein chemistry, particularly in the preparation of samples for assays and in DNA/gold nanoparticle conjugation studies.
Synonyms: EDTA
Ethylenediaminetetraacetic acid (EDTA) is a metal chelator that forms stable complexes with bivalent and trivalent metal cations, including calcium. This compound exhibits various biological activities, such as antibacterial, anti-inflammatory, antioxidant, and anticoagulant effects, and is effective in reducing metal ion-catalyzed oxidative damage to proteins. EDTA is utilized in research applications related to liver fibrosis, coronary artery disease, and neurological disorders, facilitating the maintenance of a reducing environment during protein purification.
Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO) is used in mammalian cell lysates or tissue extracts to increase protein stability.
For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.
