Biochemical Assay Reagents

Cat. No.

A87358

CAS No.

488-43-7

D-Glucamine, 98%

Synonyms: 1-Amino-1-deoxy-D-glucitol; 98%

D-Glucamine is an amino sugar derivative that functions primarily as a substrate for sugar transporter studies. Its primary application lies in pharmaceutical formulations as an excipient, facilitating the delivery of active ingredients. Additionally, D-Glucamine can be utilized in research investigating metabolic pathways and cellular transport mechanisms associated with sugars.

Cat. No.

A87359

CAS No.

1919-91-1

Naphthol AS-BI phosphate

Naphthol AS-BI phosphate serves as a substrate for both acid and alkaline phosphatases, facilitating enzymatic activity assessment in various biological contexts. This compound is particularly useful in fluorescence assays, allowing for sensitive detection and quantification. It is a valuable reagent in life science research, contributing to studies involving enzyme kinetics and cellular signaling pathways.

Cat. No.

A87368

CAS No.

74299-50-6

2'-Deoxyadenosine 5'-triphosphate disodium

Synonyms: 5'-Dexoadenosine triphosphate disodium

2'-Deoxyadenosine 5'-triphosphate disodium is a nucleotide that serves as a crucial substrate for various polymerases, including DNA polymerases and reverse transcriptases. This compound plays a vital role in DNA synthesis and reverse transcription processes. Its key biological activities make it an essential reagent for in vitro reactions in molecular biology research, facilitating the study of nucleic acid interactions and enzyme functions.

Cat. No.

A87389

CAS No.

7493-95-0

4-Nitrophenyl α-D-galactopyranoside

Synonyms: PNP-alpha-D-Gal; PNP-α-D-Gal

4-Nitrophenyl α-D-galactopyranoside serves as an artificial substrate for the detection of α-galactosidase activity. This compound facilitates the release of 4-nitrophenol (pNP) upon enzymatic cleavage, allowing for quantitative measurement of enzyme function. It is widely utilized in biochemical assays and research applications focused on glycosidase activity, contributing to studies in enzymology and carbohydrate metabolism.

Cat. No.

A87393

CAS No.

102680-35-3

2-Butenoyl coenzyme A lithium

2-Butenoyl coenzyme A lithium serves as a substrate and inactivator of Plasmodium falciparum enoyl-β-hydroxyacyl-acyl carrier protein (ACP) reductase, as well as other enoyl-CoA reductases. This compound interacts with short-chain and medium-chain CoA dehydrogenases and glutaryl-CoA dehydrogenase but exhibits no activity against wild-type isovaleryl-CoA dehydrogenase. In addition to its role in the L-isoleucine catabolic pathway, 2-Butenoyl coenzyme A lithium is useful for studying 3-ketothiolase activity and has relevance in research focused on 3-ketothiolase deficiency.

Cat. No.

A87436

CAS No.

3287-99-8

Benzylamine hydrochloride

Synonyms: Phenylmethylamine

Benzylamine hydrochloride is a test substrate for benzylamine oxidase and monoamine oxidase B. This compound is utilized in biochemical assays to investigate the enzymatic activity of these oxidases, which play a significant role in the metabolism of biogenic amines. Its applications extend to various research domains, including neurochemistry and pharmacology, aiding in the study of neurotransmitter dynamics and potential therapeutic targets.

Cat. No.

A87462

CAS No.

65147-21-9

Pro-Phe-Arg-AMC

Pro-Phe-Arg-AMC is a synthetic peptide substrate designed for the study of cysteine peptidases (CPs). It serves as a valuable tool in biochemical assays for measuring CP activity, enabling researchers to investigate enzyme kinetics and inhibition. This substrate is particularly useful in characterizing the function of CPs in various biological processes and disease models.

Cat. No.

A87547

CAS No.

911-77-3

N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride

N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride is a biochemical assay reagent that serves as a substrate for trypsin-like proteases. Its primary mechanism involves cleavage by serine proteases, leading to colorimetric detection, which facilitates enzyme activity analysis. This compound is widely used in studies of protease activity and in various biochemical assays to monitor enzymatic reactions in life sciences research.

Cat. No.

A87572

CAS No.

2963-78-2

Butyrylcholine chloride

Butyrylcholine chloride is a biochemical assay reagent that serves as a substrate for butyrylcholinesterase. This compound is essential for investigating cholinergic signaling pathways and the enzymatic activity associated with neurotransmitter regulation. It is widely utilized in research applications focusing on neurobiology, pharmacology, and toxicology, facilitating studies on enzyme kinetics and cholinergic receptor interactions.

Cat. No.

A87613

CAS No.

62802-38-4

5-Bromo-2-fluoropyrimidine

5-Bromo-2-fluoropyrimidine is a polyhalogenated pyrimidine that serves as a chemoselective amination substrate. This compound undergoes selective amination at the C-2 position, targeting the C-F bond, while preserving the C-Br bond at the C-5 position when utilized in a Cu(II)/PTABS catalytic system. It is widely applied in the synthesis of novel nitrogen-containing heterocycles and can be employed in various chemical research applications involving pyrimidine derivatives.

Cat. No.

A87620

CAS No.

9013-95-0

Levan from Erwinia herbicola, 98%

Levan from Erwinia herbicola, 98% is a polysaccharide that serves as a test substrate for levansucrase activity assays. This compound is utilized in enzymatic studies to evaluate the hydrolysis and synthesis capabilities of levansucrase. Its pure form ensures accurate and reproducible results in research applications focused on carbohydrate-active enzymes and polysaccharide biosynthesis.

Cat. No.

A87633

CAS No.

129784-87-8

1-Oleoyl-sn-glycerol

1-Oleoyl-sn-glycerol primarily acts as a biochemical assay reagent, serving as a substrate for various enzymatic reactions. It is utilized in studies related to lipid metabolism and signaling pathways, enabling researchers to investigate the role of glycerolipids in cellular processes. Its application extends to the assessment of enzyme activities and the exploration of lipid-mediated effects in physiological and pathological conditions.

Cat. No.

A87648

CAS No.

10231-84-2

p-Nitrophenyl α-L-fucoside

Synonyms: PNP α-L-Fuc

p-Nitrophenyl α-L-fucoside is a specific substrate for α-L-fucosidase, enabling the quantitative determination of enzymatic activity. This compound is widely utilized in biochemical assays to study the metabolism of fucose-containing glycoconjugates. Its use in α-L-fucosidase assays aids in the investigation of various biological processes and diseases linked to fucosylation.

Cat. No.

A87671

CAS No.

1453-82-3

Isonicotinamide

Isonicotinamide is a biochemical reagent that primarily functions as a substrate in various enzymatic reactions. It plays a critical role in biochemical assays and is widely utilized in life science research for studying metabolic processes and enzymatic regulation. Its utility extends to investigations of nicotinamide derivatives and their biological implications.

Cat. No.

A87680

CAS No.

822-67-3

2-Cyclohexen-1-ol

2-Cyclohexen-1-ol serves as a biochemical reagent and an oxidation substrate for the mouse aldo-keto reductase AKR1C12. It exhibits a Km of 1080 μM and a Kcat of 13 min-1, indicating low catalytic efficiency. This compound is valuable for studies involving enzyme kinetics and metabolic pathways related to aldo-keto reductases.

Cat. No.

A87760

CAS No.

16232-91-0

(2R)-Glycerol-O-β-D-galactopyranoside

Synonyms: 3-O-β-D-Galactopyranosyl-sn-glycerol

(2R)-Glycerol-O-β-D-galactopyranoside serves as a substrate for β-galactosidase, the lactose transporter, and thiogalactoside transacetylase. It is utilized in research applications targeting the lac operon, facilitating the study of lactose metabolism and enzymatic activity. Its effective interaction with these components enables detailed investigations into carbohydrate processing and gene expression regulation in microbial systems.

Cat. No.

A87763

CAS No.

56217-82-4

DL-Leucyl-DL-phenylalanine

DL-Leucyl-DL-phenylalanine is a dipeptide that serves as a substrate for dipeptidase enzymes. It is utilized in gel-based assays to localize and assess dipeptidase activity in Drosophila simulans and Drosophila melanogaster. This reagent is essential for studying enzymatic function and metabolic processes involving dipeptides in various biological contexts.

Cat. No.

A87809

CAS No.

63407-54-5

2-Phenylethyl β-D-thiogalactoside

Synonyms: 2-Phenylethyl 1-thio-β-D-galactoside

2-Phenylethyl β-D-thiogalactoside is a biochemical assay reagent that serves as a substrate for galactosidase enzymes. This compound is utilized in various cellular and molecular biology applications, particularly for studying enzyme kinetics and gene expression. Its ability to release phenylethyl alcohol upon hydrolysis makes it valuable for examining cellular processes and metabolic pathways related to carbohydrate metabolism.

Cat. No.

A87816

CAS No.

15673-79-7

Ribose-5-phosphate barium salt

Ribose-5-phosphate barium salt is a crucial organic compound that serves as a substrate in various biochemical pathways, particularly in nucleotide and coenzyme biosynthesis. This reagent is widely utilized in biochemical research as a precursor for generating phosphorylated sugars and engages in reactions catalyzed by enzymes within the pentose phosphate pathway. Additionally, it can function as an energy source for specific microorganisms, supporting a range of experimental applications.

Cat. No.

A87923

CAS No.

2996069-91-9

BGSN3

BGSN3 is a benzyl-guanine substrate targeted for use with SNAP-tag and H5 enzymes, demonstrating reasonable enzymatic activity with IC50 values of 17.8 µM and 10 µM, respectively. This compound functions as a click chemistry reagent, featuring an azide group that enables copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-containing molecules. Additionally, BGSN3 can participate in strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules that possess DBCO or BCN groups, making it a versatile tool for biochemical research and labeling applications.

Cat. No.

A87970

CAS No.

102185-33-1

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium acts as a substrate for phosphatase assays in biochemical research. This compound is hydrolyzed by phosphatases to produce a yellow-colored product, which can be quantified using spectrophotometric or fluorometric techniques. Additionally, it serves as a valuable reagent in enzyme-catalyzed reactions and facilitates the investigation of signal transduction pathways.

Cat. No.

A88025

CAS No.

54571-66-3

Methyl DL-pyroglutamate

Methyl DL-pyroglutamate is a biochemical assay reagent that serves as a substrate for various enzymatic reactions. This compound plays a critical role in studies of amino acid metabolism and neurotransmitter synthesis. Its application extends to investigating biochemical pathways in cell signaling and metabolic processes, making it useful in both basic and applied research settings.

Cat. No.

A88055

CAS No.

2494-56-6

Butyrylcholine iodide

Butyrylcholine iodide is a biochemical assay reagent that functions as a substrate for cholinesterases, facilitating the study of enzyme activity. Its hydrolysis produces butyric acid and choline, making it valuable for exploring cholinergic signaling pathways and neurochemical research. This compound is widely used in assays to evaluate the effects of inhibitors or activators on cholinesterase activity, contributing to advancements in pharmacology and neurobiology.

Cat. No.

A88168

CAS No.

4043-96-3

Sodium bis(4-nitrophenyl) phosphate

Sodium bis(4-nitrophenyl) phosphate serves as a biochemical assay reagent, primarily functioning as a chromogenic substrate for alkaline phosphatase. Upon dephosphorylation, it releases 4-nitrophenol, which can be measured spectrophotometrically, allowing for quantification of enzyme activity. This compound is widely utilized in enzymatic assays, facilitating research in enzymology and related fields.

Cat. No.

A88177

CAS No.

40127-11-5

L-Arginine p-nitroanilide dihydrochloride, 98%

Synonyms: H-Arg-pNA 2HCl; 98%

L-Arginine p-nitroanilide dihydrochloride is a chromogenic substrate primarily targeting cathepsin H and aminopeptidases. It is utilized in biochemical assays to facilitate the study of proteolytic enzyme activity. This compound enables the detection and quantification of enzymatic activity, providing valuable insights in molecular biology and enzymology research applications.