All Dyes

Cat. No.

A24517

CAS No.

874748-20-6

Coppersensor 1

Coppersensor-1 (CS1) is a membrane-permeable fluorescent probe with picomolar affinity for Cu⁺ ions, exhibiting high selectivity over other cellular metal ions. It enables sensitive and selective detection of copper(I) in biological samples, including live cells. CS1 is suitable for imaging studies related to copper homeostasis in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.

Cat. No.

A24518

CAS No.

878557-19-8

BODIPY-Cholesterol

BODIPY-cholesterol is a lipophilic, cell-permeable fluorescent analog of cholesterol, labeled with a BODIPY fluorophore (Ex/Em: 505/515 nm). It is commonly used to monitor sterol uptake, distribution, and inter-organelle trafficking in live cells.

Cat. No.

A24533

CAS No.

115532-50-8

TMRM Perchlorate (T668)

Synonyms: T668

Rhodamine dyes are membrane-permeable, cationic fluorescent probes that selectively accumulate in mitochondria by recognizing mitochondrial membrane potential, producing bright fluorescence. At appropriate concentrations, they exhibit low cytotoxicity and are widely used for mitochondrial staining in animal cells, plant cells, and microorganisms.

Cat. No.

A24534

CAS No.

74515-25-6

Laurdan

Laurdan is a membrane-permeable fluorescent probe that exhibits spectral sensitivity to the phospholipid phase of the membrane it integrates into. Measurement of its generalized polarization (GP) enables quantification and distinction of membrane phases, such as liquid-ordered and liquid-disordered states.

Cat. No.

A24535

CAS No.

148504-34-1

Calcein-AM

Synonyms: Calcein acetoxymethyl ester

Calcein AM is a cell-permeable, non-fluorescent dye that readily enters live cells. Once inside, it is hydrolyzed by intracellular esterases to generate Calcein, a polar and highly negatively charged molecule that is retained within the cytoplasm. Calcein emits strong green fluorescence (Ex/Em: 494/515 nm) and is commonly used in combination with Propidium Iodide for assessing cell viability and cytotoxicity.

Cat. No.

A24536

CAS No.

1447815-38-4

RhoNox-1

RhoNox-1 is a fluorescent probe designed for the specific detection of divalent iron ions (Fe²⁺). Upon reaction with Fe²⁺, RhoNox-1 forms an irreversible orange-red fluorescent product (Ex/Em: 540/575 nm). It efficiently penetrates cell membranes and is suitable for detecting intracellular Fe²⁺, with a tendency to localize in the Golgi apparatus.

Cat. No.

A24537

CAS No.

34215-57-1

DiO (DiOC18(3))

Synonyms: 3,3'-Dioctadecyloxacarbocyanine perchlorate; DiOC18(3)

DiO is a long-chain carbocyanine dye commonly used for labeling cells, organelles, liposomes, viruses, and lipoproteins. As a member of the Di-series carbocyanine dyes, DiO integrates into lipid membranes, enabling effective fluorescent tracking in various biological systems.

Cat. No.

A24538

CAS No.

115532-52-0

TMRE

Synonyms: Tetramethylrhodamine ethyl ester perchlorate

Rhodamine dyes are membrane-permeable, cationic fluorescent probes that selectively accumulate in mitochondria by sensing mitochondrial membrane potential, resulting in bright fluorescence. Due to their low cytotoxicity at appropriate concentrations, they are widely used for mitochondrial labeling in animal cells, plant cells, and microorganisms.

Cat. No.

A24539

CAS No.

217075-36-0

BODIPY 581/591 C11

BODIPY 581/591 C11 is a boron-dipyrromethene (BODIPY) derivative with excellent photostability and minimal fluorescence artifacts. It is widely used to study lipid peroxidation and antioxidant activity in living cells, and to detect ferroptosis through its reaction with hydroxyl radicals. The dye exhibits a fluorescence shift upon oxidation: the reduced form emits at 591 nm (Ex: 581 nm), while the oxidized form emits at 510 nm (Ex: 500 nm).

Cat. No.

A24540

CAS No.

154214-55-8

PKH 26

PKH26 is a red fluorescent dye that stably incorporates into the lipid regions of the cell membrane, emitting red fluorescence (Ex/Em: 551/567 nm). It is commonly used for in vitro cell labeling, cell proliferation assays, and both in vivo and in vitro cell tracking studies.

Cat. No.

A24559

CAS No.

1003197-00-9

MitoSOX Red

MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red. Excitation/emission wavelength: 510/580 nm.

Cat. No.

A24560

CAS No.

38215-36-0

Coumarin 6

Coumarin 6 is a fluorescent dye commonly used as a probe in drug delivery research. It serves as a tracer in microparticle and nanoparticle delivery systems, enabling *in vivo* tracking, cellular uptake analysis, and transport mechanism studies. Coumarin 6 exhibits strong fluorescence with excitation and emission maxima at λexc = 450 nm and λem = 505 nm, respectively, making it an ideal marker for evaluating drug distribution and release profiles in biological systems.

Cat. No.

A24570

CAS No.

41085-99-8

DiI

Synonyms: DiIC18(3)

DiI is a long-chain lipophilic carbocyanine dye commonly used for fluorescent labeling of biological membranes. Due to its strong affinity for lipid bilayers, DiI efficiently integrates into cell membranes, making it ideal for tracing cells, organelles, liposomes, viruses, and lipoproteins. It exhibits bright fluorescence, high photostability, and minimal cytotoxicity, enabling long-term imaging and tracking in both fixed and live-cell applications.

Cat. No.

A24579

CAS No.

7385-67-3

Nile Red

Synonyms: Nile Blue A oxazone; Phenoxazone 9

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.

Cat. No.

A24580

CAS No.

605-65-2

Dansyl chloride (DNSCl)

Synonyms: DNSCl

Dansyl chloride is a fluorescent labeling reagent that reacts with primary and secondary amines to form stable blue or blue-green fluorescent sulfonamide adducts. It readily labels aliphatic and aromatic amines through reaction with amino groups, making it a valuable tool for biochemical analysis. Dansyl chloride is widely used for amino acid derivatization, protein sequencing, and amino acid composition analysis, enabling sensitive fluorescence-based detection and quantification of amine-containing biomolecules.

Cat. No.

A24581

CAS No.

1934-21-0

Tartrazine

Synonyms: Acid Yellow 23; FD&C Yellow No. 5

Tartrazine (Acid Yellow 23; FD&C Yellow No. 5) is an orally active synthetic azo dye that appears as an orange-yellow, water-soluble powder producing a bright yellow coloration in solution. It is widely used as a food colorant and in pharmaceuticals and cosmetics due to its high chemical and photostability. Beyond its industrial applications, Tartrazine has been reported to induce mitochondria-mediated apoptosis and to exhibit potential biological effects, including neurodevelopmental toxicity, cytotoxicity, and genotoxicity under certain experimental conditions. It serves as a model compound in toxicological and biochemical studies investigating the safety and cellular impact of synthetic food dyes.

Cat. No.

A24582

CAS No.

75350-46-8

Fluorescein-5-maleimide

Synonyms: N-(5-Fluoresceinyl)maleimide

Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a thiol-reactive fluorescent dye widely used for covalent labeling of proteins, peptides, and other biomolecules containing cysteine residues. By forming stable thioether bonds with sulfhydryl groups, it enables precise detection and quantification of thiol-containing targets. Fluorescein-5-maleimide is employed to monitor the redox state of thiols in eukaryotic cells, label peptides or actin for protein–protein interaction studies—such as investigations of actin binding to cardiac myosin-binding protein C (cMyBP-C)—and to detect negatively charged nanoparticles. It also serves as a tool for screening mutant proteins containing cysteine residues. The dye exhibits strong fluorescence with excitation and emission maxima at 494 nm and 519 nm, respectively, making it highly suitable for fluorescence microscopy, flow cytometry, and biochemical assays.

Cat. No.

A24583

CAS No.

148757-94-2

AQC

Synonyms: 6-Aminoquinolyl-N-hydroxysccinimidyl carbamate

AQC (6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate) is a highly sensitive derivatization reagent used for amino acid and peptide analysis by high-performance liquid chromatography (HPLC) with fluorescence detection. It reacts rapidly with both primary and secondary amino groups to form stable fluorescent derivatives, enabling detection of amino acids at sub-picomolar concentrations. Due to its high reactivity, stability, and strong fluorescence yield, AQC is widely applied in protein sequencing, amino acid quantification, and metabolic profiling, providing exceptional sensitivity and reproducibility in analytical and biochemical studies.

Cat. No.

A24584

CAS No.

1593024-78-2

WSP-5

WSP-5 is a selective fluorescent probe designed for rapid detection of hydrogen sulfide (H₂S) in biological samples and live cells. It operates via a reaction-based “fluorescence turn-on” mechanism, in which WSP-5 reacts specifically and swiftly with H₂S to generate a highly fluorescent product. The probe exhibits excitation and emission maxima at 502 nm and 525 nm, respectively. Due to its excellent selectivity, sensitivity, and biocompatibility, WSP-5 is widely used in studies of H₂S biology, cellular signaling, and redox homeostasis.

Cat. No.

A24585

CAS No.

36877-69-7

Rhodamine B isothiocyanate

Rhodamine B isothiocyanate (RBITC) is a reactive fluorescent dye widely used for covalent labeling of biomolecules in imaging and analytical applications. The isothiocyanate functional group readily reacts with primary amines on proteins, peptides, and other biomolecules to form stable thiourea linkages, enabling durable fluorescent tagging. RBITC emits bright orange-red fluorescence upon green light excitation, providing strong signal intensity and excellent photostability for visualization in complex biological systems. Owing to its high sensitivity, chemical stability, and compatibility with diverse assay conditions, RBITC is extensively utilized in cell biology, immunology, neurobiology, and fluorescence microscopy studies for tracking molecular localization and dynamics.

Cat. No.

A24586

CAS No.

158757-84-7

BODIPY 558/568 C12

BODIPY 558/568 C12 is a fluorescent lipid probe based on the BODIPY (boron-dipyrromethene) dye structure. BODIPY dyes are small-molecule fluorophores characterized by strong ultraviolet-visible absorption, sharp fluorescence emission peaks, high quantum yields, and remarkable photostability. They are largely insensitive to environmental polarity and pH, maintaining consistent fluorescence under various physiological conditions. BODIPY 558/568 C12, due to its lipophilic alkyl chain, readily permeates cell membranes and specifically localizes to neutral lipid compartments, enabling selective staining of lipid droplets in both live and fixed cells. It serves as a reliable fluorescent marker for studying lipid metabolism, lipid droplet dynamics, and cellular lipid distribution. The dye exhibits maximum excitation and emission wavelengths at 558 nm and 568 nm, respectively.

Cat. No.

A24587

CAS No.

143556-24-5

L 012 sodium salt

L-012 sodium salt is a luminol-based chemiluminescent probe widely used for the sensitive detection of reactive oxygen and nitrogen species (ROS and RNS) in biological systems. It is particularly effective in monitoring NADPH oxidase (Nox)-derived superoxide production, providing high signal intensity and low background interference. Compared with conventional luminol, L-012 offers enhanced chemiluminescence sensitivity and stability, enabling real-time detection of oxidative and nitrosative stress in cells, tissues, and *in vivo* models. It is a valuable tool for studying redox signaling, inflammation, and oxidative stress–related pathophysiology.

Cat. No.

A24597

CAS No.

25535-16-4

Propidium Iodide

Synonyms: PI

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

Cat. No.

A24599

CAS No.

2031170-96-2

HKOH-1

HKOH-1 is a sensitive green fluorescent probe designed for the selective detection of hydroxyl radicals (·OH) in living cells, utilizing a maximum excitation wavelength of 500 nm and an emission wavelength of 520 nm. This reagent enables researchers to monitor oxidative stress and assess cellular responses to reactive oxygen species. Its application is critical in studies related to redox biology, neurodegenerative diseases, and cancer research.

Cat. No.

A24601

CAS No.

216434-81-0

BODIPY 493/503 methyl bromide

BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm.