Coumarin Dyes

Cat. No.

A69715

CAS No.

220001-53-6

7-Benzyloxy-4-(trifluoromethyl)coumarin

Synonyms: 7-BFC

7-Benzyloxy-4-(trifluoromethyl)coumarin (7-BFC) is a coumarin-based fluorescent substrate utilized for the evaluation of cytochrome P450 enzymes CYP1A2 and CYP3A4. Upon metabolism, 7-BFC is converted into 7-hydroxy-4-trifluoromethylcoumarin (HFC). This compound is valuable for conducting rapid screening of CYP isoform metabolism and inhibition assays in pharmacological research.

Cat. No.

A71040

CAS No.

201853-55-6

MeOSuc-Ala-Ala-Pro-Met-AMC

MeOSuc-Ala-Ala-Pro-Met-AMC is a peptide substrate specifically designed for elastase and chymotrypsin-like serine peptidases. This compound exhibits high sensitivity and specificity, enabling accurate quantification of enzymatic activity in biochemical assays. It is commonly utilized in research applications focused on proteolytic enzyme activity, contributing to studies in various fields including cancer research and inflammatory response.

Cat. No.

A71048

CAS No.

73617-90-0

Suc-Ala-Ala-Ala-AMC

Suc-Ala-Ala-Ala-AMC is a peptide substrate specifically designed for elastase or elastase-like enzymes. This compound serves as a valuable tool for studying elastase activity due to its high specificity and sensitivity. Researchers can utilize Suc-Ala-Ala-Ala-AMC in enzyme assays to investigate the role of elastase in various biological processes and disease states, making it an essential reagent for proteolytic activity analysis.

Cat. No.

A71061

CAS No.

88467-44-1

Suc-Ala-Pro-Ala-AMC

Suc-Ala-Pro-Ala-AMC is a synthetic tripeptide substrate specifically designed for elastase activity. Upon cleavage by elastase, this compound releases the fluorescent moiety AMC, enabling sensitive detection and quantification of elastase enzymatic activity. This substrate is widely used in biochemical studies to investigate elastase functions, enzyme kinetics, and the effects of inhibitors in various physiological and pathological contexts.

Cat. No.

A71064

CAS No.

113277-37-5

Suc-Ala-Ala-Pro-Val-AMC

Suc-Ala-Ala-Pro-Val-AMC is a tetrapeptide substrate specifically designed for elastase activity assays. This compound plays a crucial role in the detection and quantification of elastase activity in biological samples, making it valuable for research in enzymology and protease studies. Its specific cleavage by elastase allows for the assessment of enzyme kinetics and inhibition, facilitating studies on various physiological and pathological processes related to elastase.

Cat. No.

A76554

CAS No.

161180-11-6

7-Hydroxycoumarinyl arachidonate

Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate

7-Hydroxycoumarinyl arachidonate is a fluorogenic substrate for cytosolic phospholipase A2 (PLA2) and monoacylglycerol lipase (MAGL). Upon hydrolysis, it generates arachidonic acid (AA) and the highly fluorescent product 7-hydroxyl coumarin (7-HC). The production of 7-HC can be quantitatively assessed using a fluorometer, making this compound valuable for studies investigating the enzymatic activity of PLA2 and MAGL in various biological contexts.

Cat. No.

A76619

CAS No.

1926163-52-1

Met-Gly-Pro-AMC

Met-Gly-Pro-AMC is a fluorescent substrate specifically designed for methionine aminopeptidase 2 (MetAP2). This compound exhibits key biological activity by enabling the study of MetAP2 enzymatic activity through fluorescence detection. It is valuable in research applications focused on understanding protein processing, cellular regulation, and the role of MetAP2 in various pathological conditions.

Cat. No.

A76727

CAS No.

2741190-31-6

Coumarin–quinone conjugate

Coumarin-quinone conjugate acts as a fluorescent substrate specifically for NADH:ubiquinone oxidoreductases, featuring a coumarin fluorophore linked to a ubiquinone analog. This reagent facilitates the measurement of kinetic parameters for AIFM2/FSP1, making it valuable in research focused on ferroptosis and related metabolic processes. Its fluorescent properties enhance detection sensitivity in various biochemical assays.

Cat. No.

A77953

CAS No.

155575-02-3

Pyr-Arg-Thr-Lys-Arg-AMC

Pyr-Arg-Thr-Lys-Arg-AMC is a chromogenic substrate for proteolytic enzymes, primarily hydrolyzed by trypsin and thrombin. This peptide serves as a valuable tool for quantifying the activity of these proteases, as well as assessing the efficacy of enzyme inhibitors. Its use in biochemical assays enables researchers to explore protease function and regulation in various biological contexts.

Cat. No.

A79919

CAS No.

142997-30-6

Suc-AEPF-AMC

Suc-AEPF-AMC is a peptide substrate specifically designed for the peptidyl prolyl isomerase activity of Pin1 and Par14. This compound facilitates the study of protein folding, signaling, and regulatory mechanisms by providing a reliable substrate for enzymatic assays. Its use in research applications enhances understanding of cellular processes involving proline isomerization and protein-protein interactions.

Cat. No.

A86302

CAS No.

104086-87-5

H-Gly-Gly-Arg-AMC

H-Gly-Gly-Arg-AMC is a fluorogenic substrate designed for the continuous assessment of thrombin generation. This compound features a C-terminal 7-amino-4-methylcoumarin (AMC) moiety, which fluoresces upon cleavage. It is employed in research applications focusing on coagulation pathways and thrombin activity, enabling precise monitoring of thrombin dynamics in biological samples.

Cat. No.

A88522

CAS No.

72698-36-3

Suc-GPLGP-AMC

Suc-GPLGP-AMC is a specific peptide substrate for collagenase-like peptidases. It serves as a valuable tool for studying the enzymatic activity of these peptidases in biological research. This compound is frequently utilized in assays to monitor and quantify collagenase activity, facilitating the investigation of processes such as tissue remodeling and wound healing.

Cat. No.

A90814

CAS No.

63989-79-7

6-Aminocoumarin hydrochloride

6-Aminocoumarin hydrochloride is a coumarin dye characterized as a donor-acceptor compound, featuring an amine group as the electron donor and a carbonyl group as the acceptor. This compound exhibits fluorescence properties, making it useful in various biological and chemical assays. Its applications extend to studying cellular imaging, protein labeling, and as a fluorescent probe in biochemical research.

Cat. No.

A91554

CAS No.

23028-23-1

4-Methyl-3-phenylcoumarin

4-Methyl-3-phenylcoumarin is a biochemical reagent known for its role as a fluorescent probe. It exhibits photochemical properties that make it useful in the study of cellular processes and biomolecular interactions. This compound is valuable in various research applications, including fluorescence microscopy and biochemical assays for tracking molecular dynamics.

Cat. No.

A95519

CAS No.

53518-14-2

Coumarin 152

Coumarin 152 is a coumarin derivative with significant importance in chemical research. It is characterized by its unique structural properties and does not exhibit binding affinity towards compact myelin. This compound is useful in studies related to the synthesis of novel coumarin-based fluorophores and can serve as a valuable tool in fluorescence-based applications. Its potential applications extend to exploring biological pathways and mechanisms involving coumarin derivatives.

Cat. No.

B10983

CAS No.

95633-27-5

7-Azido-4-methylcoumarin

7-Azido-4-methylcoumarin operates primarily as a fluorescent probe for hydrogen sulfide (H2S). This compound is utilized for its high sensitivity in assessing cystathionine β-synthase activity. With an azide functional group, it is capable of undergoing copper-catalyzed azide-alkyne cycloaddition (CuAAc) and ring strain-promoted alkyne-azide cycloaddition (SPAAC) when interacting with alkyne-containing molecules or those featuring DBCO or BCN groups. Its applications extend to biological research exploring H2S signaling and related metabolic pathways.

Cat. No.

B10994

CAS No.

1357078-03-5

Coumarin boronic acid

Coumarin boronic acid serves as a fluorescent probe with excitation and emission wavelengths of 360 nm and 430 nm, respectively. This reagent facilitates real-time monitoring of amino acid and protein hydroxyl peroxides, thereby contributing to the understanding of oxidative stress and protein post-translational modifications. Its applications are valuable for researchers studying cellular mechanisms and redox biology.

Cat. No.

B10995

CAS No.

17695-46-4

4-Methylumbelliferyl butyrate

4-Methylumbelliferyl butyrate is a coumarin-based fluorogenic substrate primarily utilized for the identification of Moraxella catarrhalis C4-esterase. Upon enzymatic cleavage, it is converted to the highly fluorescent 4-methylumbelliferone, enabling sensitive detection and quantification of enzymatic activity. This reagent is valuable in biochemical assays and research applications involving enzyme kinetics and substrate specificity.

Cat. No.

B11006

CAS No.

2223665-74-3

Boc-Gln-Arg-Arg-AMC acetate

Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate specifically designed for the evaluation of protease activity. Upon hydrolysis, it releases 7-amino-4-methylcoumarin (AMC), a fluorescent compound that emits strong fluorescence under UV light. This property makes it an essential tool for monitoring proteolytic events in biochemical research and assays.

Cat. No.

B11045

CAS No.

41267-76-9

Coumarin 480

Synonyms: Coumarin 102

Coumarin 480, a laser dye, exhibits strong fluorescence properties, making it suitable for applications in fluorescence microscopy and spectroscopy. Its high quantum yield and stability under various conditions make it a valuable reagent for studying biomolecular interactions and cellular dynamics. Coumarin 480 is often utilized in dye-laser systems and as a tracer in various experimental setups in chemical and biological research.

Cat. No.

B11047

CAS No.

55804-65-4

Coumarin 343

Synonyms: C343

Coumarin 343 is a hydrophilic fluorescent probe primarily used for micro water pool applications. It exhibits strong fluorescence with an emission wavelength (λem) of approximately 425 nm and can be detected in the range of 425 nm to 550 nm. This compound is suitable for various biological imaging and analytical techniques, providing insights into molecular interactions and cellular processes.

Cat. No.

B11052

CAS No.

216757-33-4

Ac-IEPD-AMC

Ac-IEPD-AMC is a fluorogenic substrate designed for the quantification of protease activity. Upon hydrolysis, Ac-IEPD-AMC releases the fluorescent molecule 7-amino-4-methylcoumarin (AMC), which exhibits strong fluorescence under UV illumination. This compound is valuable for investigating proteolytic enzyme functions and can be utilized in various assays to measure protease activities in biological samples.

Cat. No.

B11234

CAS No.

219138-08-6

Z-VDVAD-AFC

Z-VDVAD-AFC is a fluorogenic substrate designed to measure the activity of cysteine protease 3 (Caspase-3). Upon hydrolysis, Z-VDVAD-AFC releases 7-amino-4-trifluoromethylcoumarin (AFC), which exhibits strong fluorescence under ultraviolet light. This property enables researchers to monitor proteolytic activity in various biological assays, facilitating studies in apoptosis, cell signaling, and disease mechanisms.

Cat. No.

B11237

CAS No.

1286479-53-5

4-Methyl-7-(2-nitrophenoxy)-2H-chromen-2-one

4-Methyl-7-(2-nitrophenoxy)-2H-chromen-2-one functions as a selective fluorescent probe for selenols, utilizing a nucleophilic aromatic substitution mechanism. This compound demonstrates the ability to accurately detect selenols in neutral aqueous environments while minimizing interference from thiols, amines, and alcohols commonly found in biological systems. Its applications include quantifying selenium levels in selenoenzymes and visualizing endogenous selenol activity in live cells.

Cat. No.

B11281

CAS No.

55804-67-6

Coumarin 334

Synonyms: Coumarin 521

Coumarin 334, also known as Coumarin 521, is a laser dye characterized by its rigid molecular structure. It exhibits strong fluorescence properties, making it suitable for applications in laser-based fluorescence spectroscopy and microscopy. This compound is particularly useful for studying various biological processes and is employed in a range of research applications involving photonics and imaging techniques.