Fluorescein Dyes

Cat. No.

A43678

CAS No.

128724-35-6

5(6)-Carboxynaphthofluorescein

5(6)-Carboxynaphthofluorescein is a pH-sensitive fluorophore known for its ability to fluoresce within an alkaline pH range. This compound serves as an effective fluorescent pH indicator, with excitation and emission wavelengths of 593 nm and 668 nm, respectively. It is particularly useful in the development of fiber-optic pH sensors and various biological and chemical research applications that require real-time pH monitoring.

Cat. No.

A43692

CAS No.

195136-58-4

2',7'-Difluorofluorescein

Synonyms: Oregon green 488

2',7'-Difluorofluorescein is a pH-sensitive fluorescent probe with a pKa of approximately 4.7. It provides ratiometric pH analysis through altered fluorescence intensity upon excitation at 488 nm, facilitating quantitative pH measurements between 2 and 7, particularly in submicron aerosol particles. This compound is notable for its ability to undergo buffer-mediated excited-state proton transfer and maintain stable fluorescence characteristics in physiological pH ranges, making it suitable for applications in fluorescent imaging, calcium indicator systems, and as a fluorescent protein label. Additionally, it demonstrates resistance to photodegradation, ensuring reliability in experimental settings.

Cat. No.

A43745

CAS No.

2436544-28-2

KMG-104AM

KMG-104AM is a membrane-permeable fluorescein-derived probe that specifically targets magnesium ions (Mg2+). This reagent facilitates intracellular imaging of magnesium in PC12 cells, enabling the visualization and study of magnesium dynamics within live cells. Its application in 3D imaging provides valuable insights into cellular magnesium homeostasis and its role in various physiological processes.

Cat. No.

A43797

CAS No.

208850-35-5

DAF-2T

Synonyms: Diaminofluorescein 2T

DAF-2T (Diaminofluorescein 2T) is a multifunctional fluorescent dye primarily utilized for imaging and analysis in biological research. It enables the observation of cell structures, tracking of biomolecules, and assessment of cellular functions. DAF-2T is suitable for a variety of applications, including differentiation of cell types, detection of biomolecules, study of tissue pathology, and monitoring of microorganisms. Additionally, it has potential use in emerging fields such as dye-sensitized solar cells and functional textile processing.

Cat. No.

A43856

CAS No.

384832-91-1

18:1 PE CF

Synonyms: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) ammonium

18:1 PE CF is a pH-sensitive fluorescent membrane probe designed for labeling phospholipid ethanolamine lipids. Featuring a fluorescein moiety, this compound exhibits robust fluorescence in its conjugated base form while being a weak acid. It is primarily utilized in studies requiring visualization of membrane dynamics, providing insights into cellular processes and lipid interactions.

Cat. No.

A43876

CAS No.

287111-44-8

5(6)-Carboxyfluorescein diisobutyrate

5(6)-Carboxyfluorescein diisobutyrate is a protected form of 5(6)-carboxyfluorescein, primarily utilized as a fluorescence pH indicator. This compound exhibits pH-sensitive fluorescence properties, making it valuable for monitoring changes in acidity or alkalinity in various biological contexts. Its applications include cellular imaging and assays that require real-time pH measurements.

Cat. No.

A43907

CAS No.

109653-47-6

Fluorescein hydrazide (3,6-Dihydroxyxanthane)

Fluorescein hydrazide (3,6-Dihydroxyxanthane) is a fluorescein dye that primarily targets reactive oxygen species (ROS) such as hydrogen peroxide (H2O2). This compound exhibits strong fluorescence properties, making it suitable for fluorophotometric assays in various biological studies. It is widely used in research applications focused on oxidative stress, cellular signaling, and ROS detection.

Cat. No.

A44301

CAS No.

124387-19-5

5(6)-CFDA

Synonyms: 5-(6)-Carboxyfluorescein diacetate; CFDA

5(6)-CFDA is a fluorescent dye that acts through passive diffusion into cells, where it is hydrolyzed by intracellular non-specific lipases into carboxyl fluorescein (CF). The carboxylate group of CF imparts a negative charge, enhancing its retention within cells compared to traditional fluorescein dyes. This property makes 5(6)-CFDA particularly useful for various biological applications such as cell tracking, apoptosis detection, and cellular viability assays in live cell imaging studies.

Cat. No.

A44311

CAS No.

63368-54-7

5-IAF

Synonyms: 5-Iodoacetamidofluorescein

5-IAF (5-Iodoacetamidofluorescein) is a fluorescent probe that selectively labels proteins and other molecules containing free thiols, particularly cysteine side chains. Its utility in labeling enables the visualization and tracking of thiol-containing biomolecules in various biological studies. This compound is instrumental in protein interaction studies and cellular imaging applications, providing insights into protein dynamics and cellular processes.

Cat. No.

A44316

CAS No.

78044-20-9

Fluorescein hydrazide

Fluorescein hydrazide is a highly sensitive fluorescent probe designed for the detection of Hg2+ and Co2+ ions through significant colorimetric and fluorometric changes. This compound is synthesized via the reaction of fluorescein with hydrazine, enabling its application in tracing metal ions in biochemical assays. Exhibiting an excitation wavelength of 508 nm and an emission wavelength of 531 nm, fluorescein hydrazide is an essential tool in environmental monitoring and biological research applications focused on metal ion detection.

Cat. No.

A44327

CAS No.

412319-45-0

6-FAM-PEG3-Azide

6-FAM-PEG3-Azide is a fluorescent dye that serves as a versatile labeling reagent for oligonucleotides. It features an azide functional group, enabling it to participate in copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-bearing molecules. Additionally, 6-FAM-PEG3-Azide can engage in strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with compounds containing DBCO or BCN groups. This dye is particularly valuable in various applications, including bioimaging and targeted delivery studies.

Cat. No.

A44384

CAS No.

151890-73-2

FAM hydrazide, 6-isomer

FAM hydrazide, 6-isomer is a hydrazide derivative of fluorescein (FAM) that targets carbonyl compounds. This compound is utilized for the selective labeling of aldehydes and ketones, making it suitable for various bioconjugation applications. Its fluorescent properties enable sensitive detection and visualization in chemical and biological research.

Cat. No.

A44392

CAS No.

887406-94-2

O-Methyl-O-(N-butylfluorescein)phosphate

O-Methyl-O-(N-butylfluorescein)phosphate is a versatile fluorescent dye targeting various biological processes. It exhibits strong fluorescence, making it suitable for applications in imaging and labeling studies. This compound is particularly useful in cellular assays and fluorescence microscopy, aiding in the visualization of biomolecular interactions and localization within live cells.

Cat. No.

A44431

CAS No.

136832-63-8

Green CMFDA

Synonyms: CMFDA

Green CMFDA is a cell-permeable fluorescent probe that serves as an effective cell tracer. It is cleaved by non-specific esterases present in live cells, resulting in the generation of fluorescein, which emits fluorescence at 514 nm upon excitation at 485 nm. This property makes Green CMFDA valuable for various research applications, including cell tracking and monitoring cellular processes in live-cell imaging studies.

Cat. No.

A44491

CAS No.

111843-78-8

5(6)-Carboxy-2',7'-dichlorofluorescein

5(6)-Carboxy-2',7'-dichlorofluorescein is a sensitive fluorescent probe with dual-targeting capabilities for studying fluorescein transport mechanisms. It demonstrates excellent properties for use in MRP2 vesicular transport assays, facilitating the assessment of drug transport and cellular efflux pathways. This compound is valuable for researchers investigating multidrug resistance and transporter function in various biological systems.

Cat. No.

A44513

CAS No.

217305-49-2

Fluorescein diphosphate tetraammonium

Fluorescein diphosphate tetraammonium is a fluorescent dye that acts as a fluorogenic substrate, generating fluorescein as a fluorescent product upon enzymatic hydrolysis. This compound is widely used in biochemical assays for the detection and quantification of phosphatase activity. Its bright fluorescence makes it a valuable tool in various research applications, including cell biology and enzyme kinetics studies.

Cat. No.

A44515

CAS No.

134869-04-8

Fluorescein Di-β-D-Glucuronide

Fluorescein Di-β-D-Glucuronide is a fluorescent probe substrate specifically designed to detect β-glucuronidase (βG) activity in intestinal bacteria. This compound enables non-invasive time-lapse optical imaging of βG activity, facilitating in vivo studies in models such as nude mice. Additionally, Fluorescein Di-β-D-Glucuronide supports quantitative analysis of bacterial βG enzyme activity and its inhibition, making it a valuable tool for microbiological research and enzyme kinetics.

Cat. No.

A44543

CAS No.

164256-07-9

5(6)-Carboxynaphthofluorescein diacetate

5(6)-Carboxynaphthofluorescein diacetate is a specific fluorescent substrate utilized in esterase assays. It is cleaved by intracellular esterases, leading to the production of red fluorescence, which can be quantitatively measured to assess enzyme activity. This compound is instrumental in various biological and biochemical research applications, including monitoring esterase activity in live cells.

Cat. No.

A44547

CAS No.

423762-14-5

C12FDGlcU

C12FDGlcU is a lipophilic analog of fluorescein di-β-D-glucuronic acid and serves as a specific probe for β-glucuronidase (GUS) detection. This reagent facilitates the visualization of GUS gene expression by entering cells and undergoing cleavage by β-glucuronidase, resulting in the release of fluorescein, which exhibits a yellow color and green fluorescence (Abs/Em: 495/518 nm). Its applications include monitoring gene expression in various biological research contexts.

Cat. No.

A44562

CAS No.

335193-91-4

N-Butylfluorescein

N-Butylfluorescein is an alkyl-substituted fluorescein compound that acts as a fluorescent probe. It is primarily utilized to synthesize fluorogenic substrates for the assay of phosphatidylinositol-specific phospholipase C. Its applications extend to studying enzyme activity and facilitating imaging in biological research, making it a valuable tool in cell signaling studies.

Cat. No.

A44565

CAS No.

129787-66-2

Fluorescein Di-β-D-Glucopyranoside

Fluorescein Di-β-D-Glucopyranoside acts as a specific substrate for β-glucocerebrosidase. This fluorogenic compound is primarily utilized in studies involving lysosomal enzyme activity, particularly intralysosomal β-galactosidase. Its structural properties enable effective detection and quantification of enzyme activity, making it a valuable tool in enzyme assays and research related to lysosomal storage disorders.

Cat. No.

A44581

CAS No.

632-69-9

Acid Red 94 sodium

Acid Red 94 sodium is a synthetic fluorescein derivative that functions as a photosensitizer. Its primary biological activities include antitumor effects and the inhibition of Friend Leukemia Virus (FLV) infection through photodynamic mechanisms. Additionally, this compound has demonstrated capability in inhibiting Aβ aggregation upon light irradiation. Acid Red 94 sodium is applicable in research focused on cancer therapy, viral infections, and neurodegenerative diseases.

Cat. No.

A44651

CAS No.

596-03-2

4',5'-Dibromofluorescein

Synonyms: Eosinic acid; Solvent Red 72

4',5'-Dibromofluorescein is a fluorescein derivative that serves as an effective ligand for probing protein properties through spectral analysis. Exhibiting distinctive fluorescence characteristics, it is widely utilized in biochemical research to investigate protein interactions and conformational changes. This compound's application in fluorescence microscopy and spectroscopy facilitates the study of a variety of biological processes.

Cat. No.

A44721

CAS No.

2183440-65-3

FAM hydrazide, 6-isomer hydrochloride

FAM hydrazide, 6-isomer hydrochloride is a hydrazide derivative of fluorescein, a member of the xanthene dye family. This compound serves as a useful labeling agent for carbonyl-containing compounds, including aldehydes and ketones. Its distinctive fluorescent properties make it valuable in various biological and chemical research applications, particularly in the studies involving biochemical pathways and molecular interactions.

Cat. No.

A44783

CAS No.

254109-22-3

DAF-FM DA

Synonyms: Diaminofluorescein-FM diacetate

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe specifically designed for the detection and bioimaging of nitric oxide (NO) in live cells. This compound efficiently penetrates the plasma membrane and is hydrolyzed by intracellular esterases, yielding the fluorescent DAF-FM (Ex/Em=495/515 nm). DAF-FM DA is widely used in studies exploring NO signaling pathways and its role in various physiological and pathological processes.