Fluorescein Dyes

Cat. No.

A44793

CAS No.

3326-34-9

5-Aminofluorescein

Synonyms: 5-AF

5-Aminofluorescein (5-AF) is a fluorescent marker that exhibits strong fluorescence properties, covalently attached to human serum albumin. With an excitation wavelength of 495 nm and an emission wavelength of 535 nm, this reagent is widely used in biological imaging applications. Its ability to enhance visualization in various assays makes it an invaluable tool for cellular and molecular biology research.

Cat. No.

A44803

CAS No.

51649-83-3

6-Aminofluorescein

Synonyms: 6-AF

6-Aminofluorescein is a fluorescent labeling reagent widely employed as a marker in various biological applications. Its distinctive fluorescence properties, with an excitation/emission maximum of 490/515 nm, facilitate the labeling of nanostructures such as fullerene-based liposome nanocarriers, commonly referred to as 'buckysomes.' This compound is instrumental in studies involving cellular imaging, drug delivery systems, and other fluorescence-based assays in chemical and biological research.

Cat. No.

A44806

CAS No.

254109-20-1

DAF-FM

DAF-FM is a cell-impermeant diaminofluorescein compound specifically designed as a fluorescent probe for the detection of nitric oxide (NO) in biological samples. Its excellent pH tolerance allows for effective imaging in various physiological conditions. DAF-FM is widely utilized in research applications involving NO signaling pathways, providing researchers with a valuable tool for studying nitric oxide's role in cellular processes.

Cat. No.

A44829

CAS No.

79955-27-4

5-CFDA

Synonyms: 5-Carboxyfluorescein diacetate

5-CFDA is a fluorescent dye that enables cellular imaging through its mechanism of free diffusion into cells. Once inside, it is hydrolyzed by intracellular non-specific lipases to yield carboxyl fluorescein (CF), which bears an additional negative charge, enhancing its cellular retention compared to standard fluorescein dyes. This compound is widely used in biological research for tracing cellular uptake and monitoring cellular activity in various applications.

Cat. No.

A44836

CAS No.

1043865-37-7

CO probe 1

CO Probe 1 is a highly effective fluorescent carbon monoxide (CO) probe designed for real-time imaging applications. It operates by utilizing the Tsuji-Trost reaction, where CO reduces Pd2+ to Pd0 in the presence of PdCl₂, resulting in the removal of the allyl protecting group and subsequent release of fluorescein. This process allows for a significant fluorescence enhancement of up to 150 times within 20 minutes, exhibiting high selectivity and rapid response with minimal cytotoxicity. CO Probe 1 is particularly valuable for investigating pathological mechanisms associated with CO signaling regulation in conditions such as inflammation, vascular disease, and cancer.

Cat. No.

A44861

CAS No.

51306-35-5

5-DTAF

5-DTAF is a fluorescein-based fluorescent dye primarily used for biomolecular labeling. It covalently reacts with polysaccharides and various alcohols in aqueous solutions at pH levels above 9. With an emission maximum at 518 nm upon excitation at 488 nm, 5-DTAF is ideal for applications in protein detection and cell imaging, facilitating the visualization of biomolecules in research studies.

Cat. No.

A44874

CAS No.

76863-28-0

Fluorescein-5-thiosemicarbazide

Fluorescein-5-thiosemicarbazide is a fluorescent probe featuring an amine functionality, primarily utilized for the labeling of saccharides and protein carbonyl derivatives. This compound exhibits strong fluorescent properties, making it suitable for various biological applications including cellular imaging and the study of glycan interactions. Its ability to detect and visualize specific biomolecules enhances research in biochemistry and molecular biology.

Cat. No.

A44889

CAS No.

1396969-23-5

5-FITC tyramide

5-FITC tyramide is a green fluorescent dye that combines fluorescein isothiocyanate (FITC) with tyramide, exhibiting excitation at 488 nm and emission at 525 nm. This compound serves as a reporter substrate in horseradish peroxidase (HRP)-catalyzed processes, facilitating tyramide signal amplification (TSA). 5-FITC tyramide is particularly effective in multiplex immunohistochemistry (mIHC), enabling enhanced sensitivity and specificity in detecting multiple antigens within tissue samples.

Cat. No.

A44910

CAS No.

787632-00-2

5-FAM maleimide

5-FAM maleimide is a fluorescein derivative that serves as a reactive labeling agent. This compound facilitates the conjugation of fluorescent tags to thiol-containing biomolecules, enabling sensitive detection in various applications. Ideal for labeling non-transduced cells, 5-FAM maleimide is useful in fluorescence microscopy, flow cytometry, and other analytical techniques where visualization and tracking of cellular components are essential.

Cat. No.

A44922

CAS No.

480439-15-4

Fluorescein O-methacrylate

Synonyms: Methacryloyloxy fluorescein

Fluorescein O-methacrylate is a pH-responsive fluorescent monomeric dye that exhibits fluorescence activation in alkaline environments and quenching in neutral or acidic conditions. This reversible fluorescence switching is achieved by manipulating the pH of the solution, making it a valuable tool in biological applications. As a monomer, it facilitates the synthesis of amphiphilic fluorescein polymers, which can self-assemble into stable polymer nanoparticles. These nanoparticles demonstrate enhanced fluorescence in aqueous phases and allow for the conjugation with amine-containing biomolecules, supporting sensitive optical detection of biomarkers (Ex/Em = 490/520 nm).

Cat. No.

A44929

CAS No.

1000788-53-3

DSPE-PEG-FITC (MW 3400)

DSPE-PEG-FITC (MW 3400) is a fluorescent lipid that facilitates targeted drug delivery through the formation of liposomes. Its modification with fluorescein allows for effective staining of cells, tissues, biomarkers, or nanoparticles, making it a valuable tool in cell imaging and tracking applications. This reagent is instrumental in research areas focused on drug delivery systems and fluorescence-based assays.

Cat. No.

A44936

CAS No.

2374144-21-3

FITC-hyodeoxycholic acid

FITC-hyodeoxycholic acid is a fluorescently labeled derivative of hyodeoxycholic acid, conjugated with fluorescein isothiocyanate (FITC). This compound serves as a valuable tool for investigating the biological effects and mechanisms of action of hyodeoxycholic acid. Its fluorescence property facilitates studies in cellular uptake, receptor binding, and metabolic pathways, making it suitable for applications in pharmacology, biochemistry, and molecular biology research.

Cat. No.

A44947

CAS No.

1394821-44-3

Fluorescein-CM2

Fluorescein-CM2 is a fluorogenic molecule designed for the detection of esterase activity, targeting protein-protein interactions in E. coli. This compound enables rapid screening of esterase cut sites, facilitating studies on enzymatic processes and biomolecular interactions. Its fluorescent properties serve as a valuable tool for researchers investigating the dynamics of protein interactions and the role of esterases in various biological contexts.

Cat. No.

A44962

CAS No.

2228857-33-6

Fluorescein-maleimide

Synonyms: 5-FITC-Maleimide

Fluorescein-maleimide is a thiol-reactive fluorescent derivatization reagent that specifically labels protein thiol groups through covalent binding. This compound effectively interacts with protein amino and imidazole groups under neutral pH, making it suitable for diverse biological applications. It is widely used for the fluorescent labeling of proteins, nucleic acids, or other molecules containing one or more thiol groups, with an excitation/emission peak at 494/519 nm, facilitating visualization and analysis in various research settings.

Cat. No.

A44964

CAS No.

97744-44-0

Dibenzylfluorescein

Synonyms: DBF

Dibenzylfluorescein is a fluorogenic probe that serves as a substrate for specific cytochrome P450 isoforms, notably CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). This compound, with optimal usage near its Km value of 0.87-1.9 μM (Ex=485 nm, Em=535 nm), is instrumental in assessing alterations in CYP catalytic activity resulting from pharmaceuticals or pathological conditions. Dibenzylfluorescein is widely utilized in drug metabolism studies and toxicity assessments, providing insights into enzyme function in biochemical and pharmacological research.

Cat. No.

A44967

CAS No.

7262-39-7

Fluorescein O,O′-diacrylate

Synonyms: Diacryloyloxyfluorescein

Fluorescein O,O′-diacrylate is a fluorescent compound primarily utilized for its exceptional biocompatibility and cell labeling capabilities. This reagent plays a crucial role in biological imaging and cell tracking applications, effectively labeling cells to monitor dynamic changes in vivo. Additionally, it is employed in compound delivery systems to enhance localization accuracy and improve the inhibitory effects of therapeutic agents. Furthermore, Fluorescein O,O′-diacrylate is instrumental in the preparation of polymer materials, augmenting their optical properties and biological interactions.

Cat. No.

A44974

CAS No.

217190-09-5

BDY FL-X, SE

BODIPY FL-X, SE is a fluorescent green dye specifically designed for labeling amine groups. It exhibits a high fluorescence quantum yield and is relatively insensitive to pH variations, making it a versatile tool in fluorescence applications. This dye serves as an effective alternative to commonly used labels such as FAM, Cy2, and FITC, with an absorption maximum at 504 nm and an emission maximum at 510 nm. BODIPY FL-X, SE is suitable for a variety of research applications, including cellular imaging and biomolecular interactions.

Cat. No.

A44991

CAS No.

209540-67-0

FDGlcU

FDGlcU is a fluorescent probe designed for non-invasive imaging applications. This compound exhibits strong fluorescent activity when cleaved from its non-fluorescent form, which consists of fluorescein conjugated with two mono-glucuronides (Ex/Em=480/514 nm). Its ability to selectively switch on fluorescence allows for precise visualization of biological processes in living systems, making it suitable for research in cellular and molecular biology.

Cat. No.

A45036

CAS No.

18861-78-4

Fluorescein-6-isothiocyanate

Fluorescein-6-isothiocyanate is a fluorescent dye that primarily targets amine groups in proteins through its isothiocyanate functionality. It exhibits high binding affinity with dissociation constants (Kds) of 8.74, 2.72, and 1.88 for N-Acetyl-L-Lysine, normal mouse IgG, and 4-4-20 antibodies, respectively. This compound is widely used in imaging applications, including cellular labeling and immunohistochemistry, facilitating the detection and visualization of target biomolecules in research settings.

Cat. No.

A45050

CAS No.

7308-90-9

Fluorescein dilaurate

Fluorescein dilaurate is a fluorescent dye known for its ability to emit bright green fluorescence upon excitation. It is commonly used as a colorimetric probe in biochemical assays and imaging applications, facilitating the detection of various biological molecules and cellular components. Its high sensitivity and specificity make it a valuable tool in fluorescence microscopy and flow cytometry research.

Cat. No.

A45084

CAS No.

204697-37-0

6-Fluorescein phosphoramidite

6-Fluorescein phosphoramidite is a highly effective fluorescent dye designed for the labeling of oligonucleotides. Its unique chemical structure allows for efficient incorporation into nucleic acid sequences, providing the capacity for sensitive detection and visualization in various molecular biology applications. This reagent is ideal for use in studies involving nucleic acid quantification, gene expression, and hybridization assays.

Cat. No.

A45085

CAS No.

1689512-96-6

6-ROX hydrochloride

Synonyms: 6-Carboxy-X-rhodamine hydrochloride

6-ROX hydrochloride is a fluorescent marker primarily used for oligonucleotide labeling. It serves as a receptor coupled to the fluorophore 5-FAM, facilitating Förster resonance energy transfer (FRET) imaging applications. The compound exhibits an excitation wavelength of 568 nm and an emission wavelength of 586 nm, making it suitable for various fluorescence-based assays and techniques in molecular biology research.

Cat. No.

A45099

CAS No.

1095493-05-2

Fmoc-Lys(5-FITC)-OH

Fmoc-Lys(5-FITC)-OH serves as a fluorescent marker for polypeptides and proteins, utilizing fluorescein isothiocyanate (FITC) to label amines. This compound exhibits sensitivity to pH and Cu2+ ions, making it valuable for various biochemical applications. Its fluorescent properties facilitate the study of protein interactions, localization, and dynamics in biological systems. Researchers can employ Fmoc-Lys(5-FITC)-OH in assays requiring specific protein visualization and tracking.

Cat. No.

A45103

CAS No.

2080300-52-1

Naloxone fluorescein acetate

Naloxone fluorescein acetate is a fluorescent derivative of Naloxone, an opioid antagonist that serves as a critical antidote for reversing opioid overdose effects. This compound enables the visualization of Naloxone's pharmacological activity in biological systems through fluorescent detection. Key applications for this reagent include opioid research, overdose studies, and the development of novel therapeutic strategies in addiction and pain management.

Cat. No.

A45105

CAS No.

216309-02-3

AMCA-X SE

AMCA-X SE is a fluorescein-based dye that emits a fixed blue fluorescence upon excitation. As an NHS-activated ester, it effectively forms stable amide bonds with primary amine groups, making it suitable for labeling peptides, proteins, and oligonucleotides. This reagent is widely used in various biological assays that require fluorescent tagging. The maximum excitation and emission wavelengths are 354 nm and 442 nm, respectively.