Search by product name, CAS, target or catalog number. Press Down Arrow to reach product suggestions, Tab to move between links, and Escape to close suggestions.
USD – US Dollar✓Current currency
Account
Enzyme Activity Probes & Substrates
Browse substrates and optical reporter reagents for enzyme-activity assays. Enzyme-activated fluorogenic probes can convert a chemical transformation into a fluorescence readout.[1] Choose the exact reagent for your enzyme, sample and detection method, and distinguish fluorescent from color-based readouts. This collection does not imply that every substrate is selective for one enzyme or suitable for use in living cells.
Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2 is a fluorogenic substrate specifically designed for the matrix metalloproteinase-14 (MMP-14). This compound exhibits enhanced fluorescence upon cleavage by MMP-14, allowing for sensitive detection and quantification of enzyme activity. It serves as a valuable tool for research applications focused on understanding extracellular matrix remodeling and the role of MMP-14 in various physiological and pathological processes.
4-Methylumbelliferyl-N-acetyl-α-D-glucosaminide serves as a fluorogenic substrate specifically for the detection of α-N-acetylglucosaminidase. Upon cleavage by the enzyme, it releases a highly fluorescent product, enabling sensitive quantification in various biological samples. This reagent is valuable in enzymatic assays to study glycosylation processes and related biochemical pathways.
CUG (3-Carboxyumbelliferyl-β-D-galactopyranoside) is a fluorogenic substrate primarily used to detect β-galactosidase activity. It exhibits excitation and emission wavelengths of 386 nm and 445 nm, respectively. This compound is valuable in various biochemical assays and cellular studies to monitor enzyme activity and understand metabolic processes.
Ac-EEVVAC-pNA is a chromogenic substrate specifically designed for continuous spectrophotometric assays targeting Hepatitis C Virus (HCV) NS3 protease. The substrate sequence, EEVVAC, is derived from the cleavage junction of the HCV polyprotein, facilitating the study of protease activity. Its use in biochemical assays enables researchers to investigate HCV protease mechanisms and develop potential therapeutic strategies against the virus.
Gly-Gly-AMC is a fluorogenic peptide substrate utilized for measuring protease activity. This compound is particularly effective in evaluating the bacterial protease activities of Pseudomonas aeruginosa and Staphylococcus aureus. Researchers can leverage Gly-Gly-AMC for applications in enzymatic assays and studies focused on bacterial pathogenicity and proteolytic processes.
Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 is a fluorogenic substrate specifically designed for the activity assessment of matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-26. This reagent enables the quantification of these MMPs, facilitating research into their roles in various physiological and pathological processes, including tissue remodeling and inflammation. Its application is particularly relevant in studies involving cancer biology and cardiovascular research, where MMP activity is a key determinant of disease progression.
Purple-β-D-Gal serves as a chromogenic substrate for β-galactosidase, specifically targeting the enzymatic activity of β-galactosidase. Upon hydrolysis within cells, Purple-β-D-Gal releases free indoxyl molecules, which subsequently oxidize and dimerize, resulting in the formation of chromogenic, water-insoluble indigo precipitates. This reagent is ideal for the quantitative detection and analysis of β-galactosidase activity in various biological samples.
4-Methylumbelliferyl-β-D-galactopyranoside 6-sulfate is a fluorescent dye primarily utilized for its ability to measure enzymatic activity related to galactose-6-sulfate sulfatase. This compound plays a crucial role in the diagnosis of mucopolysaccharidosis IV A by providing a sensitive method for detecting enzyme activity. Its fluorescence properties enhance analysis in biochemical assays, making it valuable in research applications focused on lysosomal storage disorders.
H-Ala-4MβNA hydrochloride is a fluorogenic substrate specifically designed for the enzyme aminopeptidase M. This compound exhibits high sensitivity and specificity, making it an effective tool for studying enzymatic activity in biological samples. It is widely utilized in research applications focused on enzyme kinetics, proteomics, and peptide metabolism.
Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) is a chromogenic substrate specifically designed for peroxidase enzymes. Upon reaction with peroxidase and peroxide, PCNPH generates a distinct purple indamine dye, facilitating the visualization and quantification of enzymatic activity. This reagent is widely used in various biochemical assays and research applications involving oxidative stress and enzyme kinetics.
6-Chloro-3-indoxyl-α-D-mannopyranoside serves as a chromogenic substrate specifically for α-mannosidase. Upon enzymatic cleavage, it produces a distinctive salmon-colored precipitate, allowing for easy visualization. This compound is widely used in biochemical assays to study α-mannosidase activity and facilitate the identification of mannosidase-producing microorganisms.
3-Acetylumbelliferyl β-D-Glucopyranoside serves as a fluorogenic substrate specifically for β-glucosidase, facilitating the detection of enzymatic activity through fluorescence. This compound is valuable as a positive control in assays involving β-D-glucosidase, enabling researchers to evaluate enzyme function and activity in various biological systems. Its application in fluorescence-based assays enhances the understanding of β-glucosidase involvement in numerous biological processes.
4-Methylumbelliferyl Decanoate is a fluorogenic substrate primarily utilized for monitoring the hydrolytic activity of carboxylesterases. When cleaved by these enzymes, it emits fluorescence, facilitating the detection and quantification of enzyme activity in biological samples. This reagent is instrumental in studies involving drug metabolism, enzyme kinetics, and the functional characterization of esterases in various biological systems.
DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye utilized as a fluorogenic substrate for aspartyl proteinases derived from human malaria parasites. This compound exhibits enhanced fluorescence upon cleavage, making it an effective tool in studying enzyme activity and kinetics. Its applications extend to research focusing on malaria pathogenesis and the development of potential therapeutic strategies against the disease.
EDANS sodium is a fluorogenic substrate employed primarily in FRET (Förster Resonance Energy Transfer) applications, specifically as a donor in nucleic acid probes and protease assays. When paired with quenchers such as DABCYL or DABSYL, EDANS sodium enhances the sensitivity and specificity of detection methods. The compound exhibits optimal absorbance and emission wavelengths at λabs = 336 nm and λem = 490 nm, making it suitable for various biological research applications involving real-time monitoring of enzymatic reactions and nucleic acid interactions.
Ac-IETD-AMC is a fluorogenic substrate specifically designed for the detection and quantification of caspase-8 activity. This compound, which includes an acetyl (Ac) moiety, serves as an efficient tool for studying apoptosis and related pathways mediated by caspase-8 and granzyme B. Its application in research allows for precise measurement of caspase-8 activity in various biological contexts, facilitating insights into cell death mechanisms and potential therapeutic targets.
2-Chloro-4-nitrophenyl-α-D-glucopyranoside serves as a chromogenic substrate targeting enzymatic activity. It is particularly useful for assays measuring the release of 2-chloro-4-nitrophenol (CNP) from conjugated carbohydrates, making it a valuable tool in the study of glycosyltransferases. This compound can aid in understanding carbohydrate metabolism and enzyme kinetics.
D-Lys(Z)-Pro-Arg-pNA is a chromogenic substrate specifically designed for the enzyme activated protein C (APC). This reagent enables the quantification of APC activity through the release of a colored product upon substrate cleavage. It serves as a valuable tool in research focused on coagulation, thrombotic disorders, and the study of proteinase activity in various biological processes.
BMV109 is a quenched probe designed for the detection of active cathepsin proteases through fluorescence activation upon cleavage and covalent binding. This reagent enables sensitive tumor imaging by providing a real-time assessment of protease activity in the tumor microenvironment. Its specificity for cathepsin proteases makes BMV109 a valuable tool in cancer research and drug discovery applications.
PHOME is a fluorogenic substrate specifically designed for soluble epoxide hydrolase (sEH). This compound undergoes hydrolysis of the epoxy ring by sEH, resulting in fluorescence. PHOME is valuable for conducting fluorescent assays to study the activity of epoxide hydrolases in various biological contexts.
Dabcyl-YVADAPV-EDANS is a fluorogenic substrate specifically designed for the interleukin-1β converting enzyme (ICE) to monitor ICE-like protease activity. It exhibits fluorescence upon cleavage, with excitation at 360 nm and emission at 480 nm, facilitating sensitive detection. This substrate is instrumental in studying K+ deprivation-induced apoptosis in cerebellar granule neurons, making it a valuable tool for apoptosis and protease research.
NFF-3 is a selective substrate for matrix metalloproteinases MMP-3 and MMP-10, facilitating their hydrolytic activity. This peptide can also be cleaved by trypsin, hepatocyte growth factor activator, and factor Xa, making it versatile in biochemical applications. Labeling NFF-3 with a CyDye pair, such as Cy3/Cy5Q, enables fluorescence detection in cellular assays, allowing for the assessment of cellular activity and the study of extracellular matrix remodeling.
Resorufin benzyl ether is a fluorogenic substrate primarily utilized for the detection of CYP3A4 enzyme activity. This compound can be further modified with a boronate moiety to enable detection of peroxynitrite (ONOO-) through a self-immolation mechanism. The fluorescence emission occurs at 590 nm when excited between 530 and 570 nm, making it a valuable tool for studies involving enzymatic activity and oxidative stress.
Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate specifically designed for the fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) activity. This compound features Edans as the fluorescent donor and Dabcyl as the quencher at its respective ends, with a central sequence that contains a conserved motif recognized by SrtA. Dabcyl-QALPETGEE-Edans provides high sensitivity in vitro, facilitating accurate measurement of the half-maximal inhibitory concentration of SrtA for various biological research applications.
Boc-AAG-pNA is a substrate for glycine endopeptidase, facilitating the study of the enzyme's amidase activity. This compound serves as an effective tool for researchers investigating glycine endopeptidase functions and kinetics. Its use can enhance understanding of proteolytic processes and enzyme-substrate interactions in various biological contexts.
