Amyloid beta (Aβ or Abeta) is a peptide of 36-C43 amino acids that is processed from the Amyloid precursor protein. Amyloid β-Peptide (1-42) human is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer disease. Downregulates bcl-2 and increases the levels of bax. Neurotoxic.
Amyloid beta (Aβ or Abeta) is a peptide of 36-C43 amino acids that is processed from the Amyloid precursor protein. Amyloid β-Peptide (1-42) human is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer disease. Downregulates bcl-2 and increases the levels of bax. Neurotoxic.
In Vitro
Recommends the following protocol for re-suspending 100 µg of peptide into a 1 mg/mL solution:
1. Bring dried peptide to room temperature for 10 minutes.
2. Add 7.5 µL cold filter sterilized 1% Ammonium hydroxide (NH4OH) to the peptide aliquot, mix well by pipetting up and down. Take care to scrape inside lower walls of tube to ensure complete re-suspension of the film.
3. Add 92.5 µL of cold filter sterilized PBS. Optional - Centrifuge monomer 14,000 x g at 4 C for approximately 5-10 minutes and keep the supernatant to remove any material that was not fully re-suspended.
4. Keep monomer on ice and use immediately.
* While it is not recommended, depending on your application small aliquots (≤ 50 μL) can be frozen and kept for up to 2 weeks at -80°C. However, aggregate should be removed by centrifugation at 4oC upon thawing.
Store lyophilized at -20ºC, keep desiccated. In lyophilized form, the chemical is stable for 36 months. In solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
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